FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rcd-1, bam
FlyBase ID
FBig0000116521
Interaction Network
Interactions Browser links
Rcd-1 network
bam network
Reported Interactions
FBrf0238143-5.coIP.WB
Reference
Sgromo et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rcd-1 

CAF40

bait

GFP tag

Rcd-1 
bam 

Bam

prey

HA tag

bam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rcd-1 
interaction domain residue 186
mutation disrupting interaction
aa 186

V186E, coordinates relative to Rcd-1-PA

bam 
CBM domain residues 17,24,28,32
mutation disrupting interaction
aa 17,24,28,32

L17E,M24E,L28E,V32E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sgromo et al., 2018)
FBrf0238143-7.PD.MW
Reference
Sgromo et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

MBP tag

bam 
bam 
Rcd-1 

CAF40

prey
Rcd-1 
Rcd-1 
Rcd-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
CBM domain
sufficient binding region
aa 13-36
bam 
CBM domain
necessary binding region
aa 13-36
Rcd-1 
ARM domain
sufficient binding region
aa 25-291

coordinates relative to Rcd-1-PA

Rcd-1 
ARM domain residue 186
mutation disrupting interaction
aa 186

V186E, coordinates relative to Rcd-1-PA

Rcd-1 
ARM domain residues 139,146
mutation disrupting interaction
aa 139,146

Y139D,G146W, coordinates relative to Rcd-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Not1 CN9BD domain and Rcd-1 ARM domain were co-purified and used together as prey in the pull-down experiment. Human CAF40 was shown to bind bam in the absence of Not1 but this was not shown for the Drosophila ortholog, Rcd-1.

(Sgromo et al., 2018)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; preys produced produced as a recombinant fusion proteins in bacterial system.

(Sgromo et al., 2018)
FBrf0262483-22.CL.FM
Reference
Salgania et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
bam 

bam

bait

mCherry tag, fused to the plasma membrane

bam 
Rcd-1 

Caf40

prey

EGFP tag

Rcd-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
interaction domain residue 24
mutation disrupting interaction
aa 24

M24E; coordinates relative to bam-PA

Rcd-1 
interaction domain surface residue 186
mutation disrupting interaction
aa 18

V186E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

bam was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

(Salgania et al., 2024)

Source was live S2R+ cells; bait and prey produced from endogenous genes.

(Salgania et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)