Bam
fused to lexA DNA-binding domain
Pum
fused to B42 activation domain
coordinates relative to pum-PA
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Three-hybrid system: yeast lexA-BD/B42-AD, bgcn was coexpressed as a bridging factor in a yeast three-hybrid assay with bam as bait and pum as prey.
mKG tag, N-terminal fragment
mKG tag, C-terminal fragment
Source was live HEK293 cells; proteins produced from transfected constructs.
bgcn was coexpressed as a bridging factor in a fluorescence complementation assay with bam as bait and pum as prey.
FLAG tag
HA tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.
The addition of antibodies against the pum region that mediates its interaction with bam disrupted the bam-pum interaction.
