FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CSN4, bam
FlyBase ID
FBig0000104784
Interaction Network
Interactions Browser links
CSN4 network
bam network
Reported Interactions
FBrf0226469-1.coIP.WB
Reference
Pan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CSN4 

Csn4

bait

HA tag

bam 

Bam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CSN4, but not CSN5, interacts with bam.

(Pan et al., 2014)

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pan et al., 2014)
FBrf0226469-2.Y2H
Reference
Pan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

fused to GAL4 DNA-binding domain

bam 
CSN4 

Csn4

prey

fused to GAL4 activation domain

CSN4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
central region
sufficient binding region
aa 151-350
CSN4 
C-terminal region
sufficient binding region
aa 287-407
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pan et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pan et al., 2014)

In yeast two hybrid assays, the CSN4-bam interaction is stronger than the bgcn-bam interaction.

(Pan et al., 2014)
FBrf0226469-3.coIP.WB
Reference
Pan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

FLAG tag

bam 
CSN4 

CSN4

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
central region
sufficient binding region
aa 151-350
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CSN4 competes with bgcn for binding to bam in this assay.

(Pan et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pan et al., 2014)

bam coimmunoprecipitates CSN4, but not CSN5, CSN6 or CSN7 (even when CSN4 is coexpressed).

(Pan et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)