FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bam, mei-P26
FlyBase ID
FBig0000097287
Interaction Network
Interactions Browser links
bam network
mei-P26 network
Reported Interactions
FBrf0219873-2.PD.WB
Reference
Insco et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

mei-P26

bait

biotin tag

mei-P26 
mei-P26 
bam 

Bam

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
3-prime UTR
sufficient binding region
nt 94-549
mei-P26 
let-7 target sites
mutation disrupting interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from transfected S2 cell extract.

(Insco et al., 2012)

The mei-P26 3-prime UTR that binds bam also mediates translational repression in vivo.

(Insco et al., 2012)

Binding of bam to the mei-P26 3-prime UTR is specific, and is not affected by the presence of excess dj 3-prime UTR competitor.

(Insco et al., 2012)
FBrf0221101-11.CH.WB
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

neutral component
mei-P26 

Mei-P26

neutral component
mei-P26 

Mei-P26

neutral component
bam 

Bam

neutral component

HA tag

bam 

Bam

neutral component

HA tag

bam 

Bam

neutral component

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mei-P26, GFP-bgcn, Sxl and HA-bam cofractionate over a range of Superdex G-200 gel filtration fractions.

(Li et al., 2013)

Source was adult female ovaries of transgenic fly line; proteins produced from endogenous gene or tagged transgenic construct.

(Li et al., 2013)
FBrf0221101-6.coIP.WB
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait
bam 

Bam

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Li et al., 2013)
FBrf0237766-1.Y2H
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

fused to lexA DNA-binding domain

mei-P26 

Mei-P26

prey

fused to GAL4 activation domain

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
N-terminal domain
sufficient binding region
aa 1-920
mei-P26 
N-terminal domain
necessary binding region
aa 1-920
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Malik et al., 2017)

Two-hybrid system: yeast lexA-BD/GAL4-AD

(Malik et al., 2017)
FBrf0237766-3.coIP.WB
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

FLAG tag

mei-P26 

Mei-P26

prey

Myc tag

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
N-terminal domain
sufficient binding region
aa 1-920
mei-P26 
N-terminal domain
necessary binding region
aa 1-920
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2017)
FBrf0237766-5.BiFC.FM
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

GFP tag, N-terminal fragment

mei-P26 

Mei-P26

prey

GFP tag, C-terminal fragment

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
N-terminal domain
sufficient binding region
aa 1-920
mei-P26 
N-terminal domain
necessary binding region
aa 1-920
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live HEK293T cells; proteins produced from transfected constructs.

(Malik et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)