Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult female ovary cDNA expression library).

Several regions of eIF-4a can bind to bam, though the eIF-4a N-terminal region binds most strongly.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The bam-eIF-4a interaction is RNA-independent and is not affected by coexpression of bgcn.

bam can coimmunoprecipitate eIF-4a, but not other RNA binding proteins (vas, Rm62 or me31B).