Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Two bam-interacting regions were identified in bgcn.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

Two-hybrid system: yeast LexA-BD/B42 AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The bam-bgcn interaction is RNA-independent and is not affected by coexpression of eIF-4a.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Three-hybrid system: yeast lexA-BD/B42-AD, bgcn was coexpressed as a bridging factor in a yeast three-hybrid assay with bam as bait and pum as prey.

Source was live HEK293 cells; proteins produced from transfected constructs.

bgcn was coexpressed as a bridging factor in a fluorescence complementation assay with bam as bait and pum as prey.

mei-P26, GFP-bgcn, Sxl and HA-bam cofractionate over a range of Superdex G-200 gel filtration fractions.

Source was adult female ovaries of transgenic fly line; proteins produced from tagged transgenic constructs.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

In yeast two hybrid assays, the CSN4-bam interaction is stronger than the bgcn-bam interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

CSN4 competes with bgcn for binding to bam in this assay.

Myc-tagged bgcn immunoprecipitated from TAP-tut complexes purified from cells expressing tagged tut, bgcn and bam.

Sequential TAP-tut and Myc-bgcn purifications indicate that tut, bgcn and bam co-exist in the same complex. Given that direct tut-bgcn interactions are not observed in yeast two hybrid assays, bam must bridge tut and bgcn. This complex is not RNA-dependent (resistant to RNaseA treatment).

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Two-hybrid system: yeast

Pairwise yeast two-hybrid interactions between tut-bam and bgcn-bam, but not tut-bgcn.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Positive control.

Source was adult male testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.