FlyBase Allele Report: Dmel\bam[Δ59]

General Information

Symbol

Dmel\bam^Δ59

Species

D. melanogaster

Name

FlyBase ID

FBal0323841

Feature type

allele

Associated gene

Dmel\bam

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Flores et al., 2015)

Progenitor genotype

P{hsneo}bam¹

(Flores et al., 2015)

Cytology

Description

Deletion that removes all of the bam protein except the 31 most C-terminal amino acid residues. Generated by imprecise excision of P{hsneo}bam¹.

(Flores et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female semi-sterile (with bam^z3-2884)

(Flores et al., 2015)

female sterile (with bam^Δ86)

(Flores et al., 2015)

neoplasia | adult stage | female (with bam^z3-2884)

(Flores et al., 2015)

neoplasia | adult stage | female (with bam^Δ86)

(Flores et al., 2015)

Phenotype Manifest In

ovary (with bam^z3-2884)

(Flores et al., 2015)

ovary (with bam^Δ86)

(Flores et al., 2015)

Detailed Description

Statement

Reference

bam^Δ59/bam^Δ86 adult females are completely sterile display tumorigenic phenotype in ovaries ('bag of marbles' phenotype). The full female sterility is not improved upon Wolbachia pipientis infection.

bam^BW/bam^Δ59 adult females are weakly fertile with a mix of tumorous and normal egg chambers, the ovarian phenotype is significantly less severe in flies infected with Wolbachia pipientis compared to those cured of the infection and their fertility is also highly increased.

(Flores et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

bam^z3-2884/bam^Δ59 has female semi-sterile phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

bam^z3-2884/bam^Δ59 has neoplasia | adult stage | female phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

Suppressed by

Statement

Reference

bam^Δ86/bam^Δ59 has female sterile phenotype, suppressible | partially by Dsim\bam^bam.Venus

(Flores et al., 2015)

bam^z3-2884/bam^Δ59, bgcn[+]/bgcn¹ has female sterile phenotype, suppressible by Dsim\bam^bam.Venus

(Flores et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

bam^z3-2884/bam^Δ59 has ovary phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

NOT Enhanced by

Statement

Reference

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype, non-enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

Other

Statement

Reference

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype

(Flores et al., 2015)

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has female germline stem cell phenotype

(Flores et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced fertility of bam^Δ59/bam^BW females is enhanced by combination with a single copy of bgcn¹ : the females are sterile with completely tumorous ovaries.

(Flores et al., 2015)

Xenogenetic Interactions

Statement

Reference

Expression of Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} partially restores fertility of the otherwise sterile bam^Δ59/bam^Δ86 females. The ovaries of the rescue females show multiple morphological defects that accumulate with age (loss of germline stem cells, improper number of cyst divisions, mitotic synchrony defects). However, when two copies of the Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} transgene are used, the fertility of the rescue females is reduced (compared to one copy rescue) and they become virtually sterile by day 15. They also show accelerated loss of the germline stem cells from the ovaries and often lack them or any germline cells altogether. These effects are alleviated even by a single copy of endogenous wild-type bam. The partial fertility of bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue flies is also improved by Wolbachia pipientis infection.

The ovarian defects observed in bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue females cannot be exacerbated by combination with a single copy of bgcn¹. Additionally, combination with Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} suppresses the tumorous ovary phenotype characteristic for bam^BW/bam^Δ59;bgcn¹/+ double mutant flies.

(Flores et al., 2015)

Complementation and Rescue Data

Rescued by

bam^Δ86/bam^Δ59 is rescued by bam^4.1.Venus

(Flores et al., 2015)

Comments

Expression of bam^{4.1.T:Avic\GFP-YFP.Venus} restores fertility of the otherwise sterile bam^Δ59/bam^Δ86 females and bam^BG/bam^Δ86 males, although under sperm exhaustion conditions the rescue is not complete. Ovaries of the rescue females have wild-type morphology.

The fertility of bam^Δ59/bam^Δ86;bam^{4.1.T:Avic\GFP-YFP.Venus} rescue flies is neither increased nor diminished by Wolbachia pipientis infection.

(Flores et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bam^Δ59

(Wenzel and Aquadro, 2023, Wenzel and Aquadro, 2023, Bubnell et al., 2021, Flores et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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