FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bam, twin
FlyBase ID
FBig0000104039
Interaction Network
Interactions Browser links
bam network
twin network
Reported Interactions
FBrf0230176-3.coIP.WB
Reference
Fu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
twin 

Twin

bait

FLAG tag

bam 

Bam

prey

Myc tag

bam 
bam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
central region
sufficient binding region
aa 151-300
bam 
C-terminal region
sufficient binding region
aa 301-442
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fu et al., 2015)

Expression of Pop2 reduces the twin-bam interaction.

(Fu et al., 2015)
FBrf0230176-4.coIP.WB
Reference
Fu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

Myc tag

twin 

Twin

prey

FLAG tag

twin 
twin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
twin 
N-terminal region
necessary binding region
aa 51-100
twin 
N-terminal region residues 76-81
mutation disrupting interaction
aa 76-81

L76A, L79A and L81A (or L76G, L79G and L81G)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fu et al., 2015)
FBrf0230176-5.coIP.WB
Reference
Fu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
twin 

Twin

bait

FLAG tag

twin 
bam 

Bam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
twin 
N-terminal region residues 76-81
mutation disrupting interaction
aa 76-81

L76A, L79A and L81A (or L76G, L79G and L81G)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fu et al., 2015)
FBrf0234954-12.coIP.WB
Reference
Shan et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bam 

Bam

bait

TAP tag

twin 

Twin

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shan et al., 2017)
FBrf0238143-4.coIP.WB
Reference
Sgromo et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
twin 

CCR4

bait

GFP tag

bam 

Bam

prey

HA tag

bam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bam 
CBM domain residues 17,24,28,32
mutation disrupting interaction
aa 17,24,28,32

L17E,M24E,L28E,V32E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sgromo et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)