FlyBase Cell Line Report: OSC

General Information

Symbol

OSC

Species

D. melanogaster

Feature type

cultured cell-line

FlyBase ID

FBtc0000288

Source

w¹¹¹⁸; P{hs-bam.O}11d bam^Delta86 ry506 ^e1/bam^Delta86 P{ovo-lacZ.M}3.1 P{vas^T:GFP}3.1

(Saito et al., 2009)

Tissue Source

ovary

(Saito et al., 2009)

Developmental Stage

adult stage

(Saito et al., 2009)

Lab of Origin

Siomi

(Saito et al., 2009)

Mikiko Siomi

(Saito et al., 2009)

Characterization

Sex

female (based on Derived from adult ovary)

(Saito et al., 2009)

Karyotype

female

(Saito et al., 2009)

Associated Genes (1)

Integrated Constructs (0)

Comments

New stable cell line derived from this cell line: An OSC cell line stably expressing flag-tagged Spyo\Cas9 was generated. Additionally, new stable cell lines were derived from the OSC[Cas9] cell line: OSC cell lines stably expressing HA-tagged Dora, and a knockout of Dora in which a hygromycin resistance gene was inserted into its open reading frame, were generated. New stable cell lines were then generated from the Dora and Dora-KO: cell line stably expressing Rren\LUC fused to the 3'UTR of Tom.

(Akulenko et al., 2025)

New stable cell line derived from this cell line: Several stable cell lines were created in OSC cells. The lines are OSC with dPcf11-halo, OSC with Ubi-TurboID-Myc-Panx, OSC with Ubi-TurboID-Myc-Spt5, and OSC with Ubi-TurboID- Myc-YFP.

(Liu et al., 2025)

New stable cell line derived from this cell line: Endogenously tagged alleles were established for piwi, shu, Gle1, vir and Nbr in OSC cells.

(Meng et al., 2022)

New stable cell line derived from this cell line: Stable OSC lines expressing internally tagged kipf under control of the Ubi-p63E promoter and an SV40 3'UTR were generated by integration into an RMCE landing site.

(Baumgartner et al., 2022)

OSC cells were obtained from the laboratory of Mikiko Siomi.

(Fefelova et al., 2022)

New stable cell line derived from this cell line: OSC cells harboring stably integrated Tag:HA-ZsGreen tethering reporters were created.

(Eastwood et al., 2021)

New stable cell line derived from this cell line: OSC cells with ctp and egg N-terminally tagged with GFP,Tag:FLAG and Panx C-terminally tagged with GFP,Tag:FLAG at their endogenous loci were created.

(Schnabl et al., 2021)

OSC cells were obtained from the laboratory of M Siomi.

(Munafò et al., 2021)

Source of OSC cells described as gift from Mikiko Siomi in paper and DGRC in FTYP tool.

(Eastwood et al., 2021)

New stable cell line derived from this cell line: Both parental OSC and CRISPR-Cas9 generated OSC derivative with endogenous arx tagged with FLAG-HA were used.

(Mugat et al., 2020)

New stable cell line derived from this cell line: A stable OSC reporter line was generated carrying the tj enhancer driven GFP_P2A-blasticidin-resistance harboring ten intronic boxB sites and 14 upstream UAS sites; Addgene 128010.

(Batki et al., 2019)

New stable cell line derived from this cell line: Set of cell lines created stably carrying wild type and mutant constructs of Csas.

(Mertsalov et al., 2016)

vas expression not observed, consistent with follicle (somatic) cells.

(Saito et al., 2009)

Parental Lines (1)

Cloned from: