FlyBase Recombinant Construct Report: P{-898/+133-bam:GFP}

General Information

Symbol

P{-898/+133-bam:GFP}

FlyBase ID

FBtp0016718

Feature type

transgenic_transposable_element

Component Allele(s)

bam^-898+133.GFP

Also Known As

bam-GFP, P{bamP-GFP}, bamP-GFP, bamP-bam-GFP

Size

Expression Data

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele bam^-898+133.GFP

Transgenic product class

wild_type

(Chen and McKearin, 2003)

Component

Use(s)

Regulatory region(s)

bam

bam_-898-+133

Encoded product / tool

bam

Tagged with

GFP

green fluorescent protein

Description

bam promoter sequences from -898 to +133bp (relative to the transcription start site) drive expression of the bam open reading frame tagged at the C-terminal end with GFP. bam 3' UTR sequences are also present.

(Chen and McKearin, 2003)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

bam^-898+133.GFP

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

female germline cell of germarium region 2

(Chau et al., 2012)

adult stage | male & spermatogenesis

male germline stem cell

(Chau et al., 2012)

spermatogonium

(Chau et al., 2012)

oogenesis

cystoblast

(Chau et al., 2009, Li et al., 2009, Chen and McKearin, 2003)

cyst cell of ovary

(Chau et al., 2009, Chen and McKearin, 2003)

Additional Information

Statement

Reference

In females, nos protein accumulates in the germline stem cells (GSCs) located at the anterior tip of the germarium, which does not express bam. When the GSC divides, one cell remains at the tip and retains its GSC identity, whereas the other daughter moves away from the tip and initiates a differentiation program which includes significant accumulation of the bam protein and rapid down-regulation of the nos protein in region 2 of the germarium. As the cells cease to divide, the relationship between nos and bam expression inverts, culminating with high levels of nos, as bam expression is extinguished in the 16-cell cyst in region 3. By contrast, in males, nos is expressed both in the testicular GSCs and in their bam-expressing daughters.

(Chau et al., 2012)

bam and nos expression are reciprocal patterns in early germ cells.

(Li et al., 2009)

Strong reporter expression is observed in cystoblasts and young cystocytes. No expression is observed in germline stem cells.

(Chen and McKearin, 2003)

Marker for

cyst cell of ovary

(Chau et al., 2009)

cystoblast

(Chau et al., 2009)

Reflects expression of

bam

(Chau et al., 2009, Li et al., 2009, Chen and McKearin, 2003)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

-898/+133-Bam:GFP

(Chen and McKearin, 2003)

BAM-GFP

(Ji and Tulin, 2012, Liu et al., 2010)

Bam promoter-GFP

(Leatherman and DiNardo, 2008)

Bam-GFP

(Jensen et al., 2021)

Bam:GFP rescuing transgene

(Li et al., 2009)

P(bamP-gfp)

(Liu et al., 2017)

P[w⁺ Bam:GFP]

(Szakmary et al., 2005)

Pro-bam-GFP

(Schulz et al., 2004)

P{-898/+133-bam:GFP}

(Banisch et al., 2021, FlyBase, 1996-)

P{bam-GFP}

(Chau et al., 2012)

P{bamP-GFP}

(Chen et al., 2017, Chen et al., 2009, Jiang et al., 2008, Maines et al., 2007, Yang et al., 2007, Chen and McKearin, 2005)

P{bamP-gfp}

(Xia et al., 2010)

P{bamP::gfp}

(Xia et al., 2012)

P{bamp-bam:GFP}

(Ji et al., 2017)

bam-GFP

(Li et al., 2016, Matsuoka et al., 2014, Cash and Andrews, 2012, Narbonne-Reveau et al., 2006, Singh et al., 2006, Xi and Xie, 2005)

bam::GFP

(Vallés et al., 2024)

bamP-GFP

(Monteiro et al., 2023, Dolezal et al., 2015, Xin et al., 2013, Chau et al., 2009, Guo and Wang, 2009, Neumuller et al., 2008)

bamP-bam-GFP

(Harris et al., 2025, Zhang et al., 2024)

bamP-gfp

(Xia et al., 2010)

bamP: bam-gfp

(Rastegari et al., 2020)

bamP:GFP

(Banisch et al., 2021)

bamp-Bam:GFP

(Lin et al., 2020)

pBam-bam:GFP

(Bener et al., 2025)

{bamP-GFP}

(Chen and McKearin, 2003)

Secondary FlyBase IDs

References (41)

Report Sections

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