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General Information
Symbol
P{-898/+133-bam:GFP}
FlyBase ID
FBtp0016718
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
bam-GFP, P{bamP-GFP}, bamP-GFP
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele bam-898+133.GFP
Product class / Tool use(s)
Regulatory region(s)
Description

bam promoter sequences from -898 to +133bp (relative to the transcription start site) drive expression of the bam open reading frame tagged at the C-terminal end with GFP. bam 3' UTR sequences are also present.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In females, nos protein accumulates in the germline stem cells (GSCs) located at the anterior tip of the germarium, which does not express bam. When the GSC divides, one cell remains at the tip and retains its GSC identity, whereas the other daughter moves away from the tip and initiates a differentiation program which includes significant accumulation of the bam protein and rapid down-regulation of the nos protein in region 2 of the germarium. As the cells cease to divide, the relationship between nos and bam expression inverts, culminating with high levels of nos, as bam expression is extinguished in the 16-cell cyst in region 3. By contrast, in males, nos is expressed both in the testicular GSCs and in their bam-expressing daughters.

bam and nos expression are reciprocal patterns in early germ cells.

Strong reporter expression is observed in cystoblasts and young cystocytes. No expression is observed in germline stem cells.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (18)
References (32)