Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction decreased by coexpression of krz

The PLA signal develops only if primary antibodies to the two proteins are in close proximity to each other (within tens of nanometers).

Dsor1 showed an interaction with rl, but not with Axn, arr or dsh in this assay.

Source was fixed larval wing disc of transgenic fly line; proteins produced from tagged transgenic construct or from endogenous gene.