FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\shf
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General Information
Symbol
Dmel\shf
Species
D. melanogaster
Name
shifted
Annotation Symbol
CG3135
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003390
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
shifted (shf) encodes a secreted protein that positively modulates Hedgehog signaling. It controls the diffusion of the lipid-modified protein encoded by hh by stabilizing its interaction with the heparan sulfate proteoglycans. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
6C13-6D1
Sequence location
Recombination map
1-18
RefSeq locus
NC_004354 REGION:6771463..6779659
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q02936
(Gorfinkiel et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables signaling receptor binding
inferred from biological aspect of ancestor with PANTHER:PTN001029349
(GO Reference Genome Project, 2011-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in positive regulation of smoothened signaling pathway
inferred from mutant phenotype
(Avanesov and Blair, 2013, Bilioni et al., 2013, Avanesov et al., 2012, Sánchez-Hernández et al., 2012, Glise et al., 2005, Gorfinkiel et al., 2005)
involved_in protein localization to extracellular region
inferred from mutant phenotype
(Avanesov and Blair, 2013, Bilioni et al., 2013)
involved_in protein stabilization
inferred from mutant phenotype
(Gorfinkiel et al., 2005)
involved_in synaptic target recognition
inferred from mutant phenotype
(Kurusu et al., 2008)
NOT involved_in Wnt signaling pathway
inferred from mutant phenotype
(Glise et al., 2005, Gorfinkiel et al., 2005)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in extracellular matrix
inferred from direct assay
(Gorfinkiel et al., 2005)
located_in extracellular region
inferred from direct assay
(Glise et al., 2005)
inferred from direct assay
(Sánchez-Hernández et al., 2012)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cell surface
inferred from biological aspect of ancestor with PANTHER:PTN001029349
(GO Reference Genome Project, 2011-)
is_active_in extracellular region
inferred from biological aspect of ancestor with PANTHER:PTN001029349
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
shifted (shf) encodes a secreted protein that positively modulates Hedgehog signaling. It controls the diffusion of the lipid-modified protein encoded by hh by stabilizing its interaction with the heparan sulfate proteoglycans. [Date last reviewed: 2019-03-14]
Pathway (FlyBase)
POSITIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -
Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Required for normal accumulation and movement of lipid-modified hedgehog (hh) morphogen. May act by stabilizing the interaction between heparan sulfate proteoglycans (HSPGs) and hh, HSPGs being required for diffusion of hh morphogen. Not involved in wingless (wg) morphogen movement, suggesting that it may provide HSPG specificity for Hh.
(UniProt, Q9W3W5)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
*shf: shifted
Vein L3 fails to reach wing margin and is shifted toward L4. Anterior crossvein usually lacking. Wings divergent. Postscutellar bristles small and erect. Body small. Viability 60% wild type. Female often sterile. RK2.
shf2
Veins closer together than in wild type. L3 and L4 tend to fuse near anterior crossvein; anterior crossvein shortened, knotted, or absent. Phenotypic effect visible in prepupal wing bud, the two longitudinal veins diverging at a smaller-than-normal angle [Waddington, 1940, J. Genet. 41: 75-139 (fig.)]. Eyes sometimes slightly rough. Scutellar bristles often absent. Scutellum short. Wings narrow and often warped downward. Fertility and viability good. RK2.
*shf3
thumb
shf3: shifted-3
From Bridges and Brehme, 1944, Carnegie Inst. Washington Publ. No. 552: 173.
Like shf2 but more extreme. Viability about 70% wild type. Frequently infertile. RK2.
shfov: shifted-oval
Eyes rough and narrow. First basal wing cell absent because L3 and L4 veins close. Wings narrow and pointed. Viability and fertility low. RK2.
Summary (Interactive Fly)

ortholog of the human Wnt inhibitory factor - required for Hh stability and for lipid-modified Hh diffusion - colocalizes with Hh in the extracellular matrix and interacts with the heparan sulfate proteoglycans

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\shf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W3W5)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
shf-RA
FBtr0070978
NM_132121
1784
456
shf-RB
FBtr0340088
NM_001298034
1796
460
shf-RC
FBtr0340089
NM_001298035
1733
439
shf-RD
FBtr0340090
NM_001298036
1745
443
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
shf-PA
FBpp0070939
50.7
456
8.85
Q9W3W5
NP_572349
AAF46198
shf-PB
FBpp0309087
51.1
460
8.80
X2JED7
NP_001284963
AHN59434
shf-PC
FBpp0309088
49.0
439
8.95
X2JAQ0
NP_001284964
AHN59435
shf-PD
FBpp0309089
49.3
443
8.90
X2JCU9
NP_001284965
AHN59436
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with hh.

(UniProt, Q9W3W5)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\shf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.17

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
mesothoracic tergum primordium | restricted
(Glise et al., 2005)
wing pouch | restricted
(Glise et al., 2005)
wing hinge primordium
(Glise et al., 2005)
Additional Descriptive Data

shf protein has a distribution similar to that of shf transcript, and is localized to parts of the presumptive notum, to the presumptive wing hinge, and to the anterior and posterior edges of the wing pouch. The wing pouch expression is stronger at the anterior- and posterior-most edges, and more pronounced in the posterior compartment. Immunoreactivity appears to be associated with the cell surface.

(Glise et al., 2005)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in extracellular matrix
inferred from direct assay
(Gorfinkiel et al., 2005)
located_in extracellular region
inferred from direct assay
(Glise et al., 2005)
inferred from direct assay
(Sánchez-Hernández et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\shf in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of shf
Transgenic constructs containing regulatory region of shf
Aberrations (Deficiencies and Duplications) ( 13 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (28)
Hsap\WIF1
11 of 14
Yes
Yes
2  
Hsap\FBN1
2 of 14
No
No
Hsap\FBN2
2 of 14
No
No
2  
Hsap\TENM2
2 of 14
No
No
Hsap\TNC
2 of 14
No
No
Hsap\TNR
2 of 14
No
No
Hsap\DLK1
1 of 14
No
No
Hsap\DLL1
1 of 14
No
No
Hsap\DLL3
1 of 14
No
Yes
Hsap\DLL4
1 of 14
No
No
Hsap\FAT1
1 of 14
No
No
Hsap\FBN3
1 of 14
No
No
Hsap\JAG2
1 of 14
No
No
Hsap\LRP1B
1 of 14
No
No
Hsap\LRP1
1 of 14
No
No
1  
Hsap\NELL1
1 of 14
No
Yes
1  
Hsap\NELL2
1 of 14
No
Yes
Hsap\NOTCH1
1 of 14
No
No
Hsap\PEAR1
1 of 14
No
No
2  
Hsap\SLIT1
1 of 14
No
No
Hsap\SNED1
1 of 14
No
No
Hsap\SVEP1
1 of 14
No
No
Hsap\TENM1
1 of 14
No
No
Hsap\TENM3
1 of 14
No
No
Hsap\TENM4
1 of 14
No
No
Hsap\TNN
1 of 14
No
No
Hsap\TNXB
1 of 14
No
No
Hsap\VWDE
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (16)
Rnor\Wif1
11 of 14
Yes
Yes
Rnor\Fbn1
2 of 14
No
No
Rnor\Tenm2
2 of 14
No
No
Rnor\Tnr
2 of 14
No
No
Rnor\Dlk1
1 of 14
No
No
Rnor\Dll1
1 of 14
No
No
Rnor\Fat1
1 of 14
No
No
Rnor\Jag1
1 of 14
No
No
Rnor\Lrp1
1 of 14
No
No
Rnor\Nell1
1 of 14
No
No
Rnor\Nell2
1 of 14
No
Yes
Rnor\Notch4
1 of 14
No
No
Rnor\Svep1
1 of 14
No
No
Rnor\Tenm3
1 of 14
No
No
Rnor\Tnc
1 of 14
No
No
Rnor\Tnn
1 of 14
No
No
Mus musculus (laboratory mouse) (17)
Mmus\Wif1
10 of 14
Yes
Yes
Mmus\Fbn1
2 of 14
No
No
Mmus\Fbn2
2 of 14
No
No
Mmus\Tenm2
2 of 14
No
No
Mmus\Tnc
2 of 14
No
No
Mmus\Fat1
1 of 14
No
No
Mmus\Jag1
1 of 14
No
No
Mmus\Jag2
1 of 14
No
No
Mmus\Lrp1
1 of 14
No
No
Mmus\Nell1
1 of 14
No
Yes
Mmus\Nell2
1 of 14
No
Yes
Mmus\Notch3
1 of 14
No
No
Mmus\Notch4
1 of 14
No
No
Mmus\Svep1
1 of 14
No
No
Mmus\Tenm3
1 of 14
No
No
Mmus\Tnn
1 of 14
No
No
Mmus\Tnxb
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (21)
Xtro\wif1
6 of 13
Yes
Yes
Xtro\adgre2
1 of 13
No
No
Xtro\dlk1
1 of 13
No
Yes
Xtro\emx2
1 of 13
No
No
Xtro\fat1
1 of 13
No
No
Xtro\fbn1
1 of 13
No
No
Xtro\fbn2
1 of 13
No
No
Xtro\fbn3
1 of 13
No
No
Xtro\hmcn1
1 of 13
No
No
Xtro\jag1
1 of 13
No
No
Xtro\jag2
1 of 13
No
No
Xtro\lrp1
1 of 13
No
No
Xtro\megf10
1 of 13
No
No
Xtro\nell2
1 of 13
No
No
Xtro\notch1
1 of 13
No
No
Xtro\notch2
1 of 13
No
No
Xtro\pear1
1 of 13
No
No
Xtro\svep1
1 of 13
No
No
Xtro\tmod2
1 of 13
No
No
Xtro\tnn
1 of 13
No
No
Xtro\tnr
1 of 13
No
No
Danio rerio (Zebrafish) (30)
Drer\wif1
11 of 14
Yes
Yes
Drer\tenm2b
2 of 14
No
No
Drer\tnca
2 of 14
No
Yes
Drer\tncb
2 of 14
No
No
Drer\tnr
2 of 14
No
No
Drer\vwde
2 of 14
No
Yes
Drer\vwdel
2 of 14
No
Yes
Drer\dla
1 of 14
No
No
Drer\dld
1 of 14
No
No
Drer\egfl6
1 of 14
No
Yes
Drer\fat1a
1 of 14
No
No
Drer\fat1b
1 of 14
No
No
Drer\fbn2a
1 of 14
No
No
Drer\fn1b
1 of 14
No
Yes
Drer\jag1a
1 of 14
No
No
Drer\jag1b
1 of 14
No
No
Drer\jag2a
1 of 14
No
No
Drer\jag2b
1 of 14
No
No
Drer\nell2a
1 of 14
No
No
Drer\nell2b
1 of 14
No
No
Drer\notch1a
1 of 14
No
No
Drer\notch1b
1 of 14
No
No
Drer\notch3
1 of 14
No
No
Drer\si:dkey-237h12.3
1 of 14
No
No
Drer\svep1
1 of 14
No
No
Drer\tenm1
1 of 14
No
No
Drer\tenm2a
1 of 14
No
No
Drer\tenm3
1 of 14
No
No
Drer\tenm4
1 of 14
No
No
Drer\tnn
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (17)
Cele\C02B10.3
1 of 14
Yes
No
Cele\C05B5.5
1 of 14
Yes
Yes
Cele\C05B5.11
1 of 14
Yes
Yes
Cele\C23H4.8
1 of 14
Yes
No
Cele\ced-1
1 of 14
Yes
No
Cele\F21A3.3
1 of 14
Yes
Yes
Cele\F40A3.2
1 of 14
Yes
No
Cele\F46F11.7
1 of 14
Yes
No
Cele\fmi-1
1 of 14
Yes
No
Cele\glp-1
1 of 14
Yes
No
Cele\irg-7
1 of 14
Yes
Yes
Cele\slt-1
1 of 14
Yes
No
Cele\T01D3.1
1 of 14
Yes
No
Cele\T16G12.10
1 of 14
Yes
Yes
Cele\ten-1
1 of 14
Yes
No
Cele\txt-5
1 of 14
Yes
No
Cele\Y46G5A.29
1 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (13)
Agam\AgaP_AGAP000117
11 of 12
Yes
Yes
Agam\AgaP_AGAP000461
1 of 12
No
No
Agam\AgaP_AGAP007924
1 of 12
No
No
Agam\AgaP_AGAP009762
1 of 12
No
No
Agam\AgaP_AGAP009763
1 of 12
No
No
Agam\AgaP_AGAP010021
1 of 12
No
No
Agam\AgaP_AGAP010265
1 of 12
No
No
Agam\AgaP_AGAP010335
1 of 12
No
No
Agam\AgaP_AGAP010940
1 of 12
No
No
Agam\AgaP_AGAP011034
1 of 12
No
No
Agam\AgaP_AGAP011593
1 of 12
No
No
Agam\AgaP_AGAP012372
1 of 12
No
No
Agam\AgaP_AGAP012386
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\AT5G42620
1 of 13
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:shf. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (17)
Ten-m
2 of 13
C901
1 of 13
CG15861
1 of 13
CG32373
1 of 13
Delta
1 of 13
drpr
1 of 13
eater
1 of 13
LRP1
1 of 13
NimB1
1 of 13
NimB2
1 of 13
NimB4
1 of 13
NimB5
1 of 13
NimC1
1 of 13
NimC2
1 of 13
NimC3
1 of 13
NimC4
1 of 13
Ten-a
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    WIF1; Wnt inhibitory factor 1
    11 of 14
    605186
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        shf
        suppressible
        shf::Drer\wif1
        (Avanesov et al., 2012)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        fz2
        suppressible
        shf
        (Avanesov et al., 2012)
        External Data
        Subunit Structure (UniProtKB)
        Interacts with hh.
        (UniProt, Q9W3W5 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-18
        Cytogenetic map
        6C13-6D1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        6C13-6D1
        Limits computationally determined from genome sequence between P{EP}Ctr1AEP1364&P{EP}EP1445 and P{EP}C3GEP1613
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        6A3-6F11
        (Demerec et al., 1942)
        Determined by deletion analysis (details unspecified).
        (Demerec et al., 1942)
        Experimentally Determined Recombination Data
        Location

        1-18

        (FlyBase, 2016)

        1-18.9

        (Comeron et al., 2012)

        1-17

        (Bateman, 1950)

        1-17.9

        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (9)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (21)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Glise et al., 2005)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Mutants display hyperplastic phenotype, with tissue overgrowth in mitotic recombination clones.

            (Mechler, 1994)

            Mutations in shf cause extra longitudinal or transverse veins with plexated vein phenotypes. kn, fu and shf act synergistically against Px, px, net, dsr and emc.

            (Diaz-Benjumea and Garcia-Bellido, 1990)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: shf CG3135

            (Guerrero, 2004.2.25)
            Additional comments
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (13)
            Reported As
            Symbol Synonym
            CG3134
            (Gorfinkiel et al., 2005)
            CG3135
            (Ni et al., 2010.12.1, Fujikawa et al., 2009, Glise et al., 2005)
            CT10514
            (Hynes and Zhao, 2000)
            D-Wif
            (Gorfinkiel et al., 2003)
            DWif
            (Bilioni et al., 2013)
            DmWIF
            (Gorfinkiel et al., 2005)
            DmWif
            (Glise et al., 2003)
            Shf
            (Jiménez-Jiménez et al., 2024, Gradilla and Guerrero, 2022, Norman et al., 2016, Mbodj et al., 2013, Callejo et al., 2007, Wilson and Chuang, 2006, Gorfinkiel et al., 2005)
            shf
            (Pignataro et al., 2024, Deshpande et al., 2023, Benner et al., 2019, Gene Disruption Project members, 2018-, Matamoro-Vidal et al., 2018, Avanesov and Blair, 2013, Bilioni et al., 2013, Deshpande et al., 2013, Short and Lazzaro, 2013, Avanesov et al., 2012, Nfonsam et al., 2012, Sánchez-Hernández et al., 2012, Toku et al., 2011, Kong et al., 2010, Long et al., 2010, Zhai et al., 2010, Fujikawa et al., 2009, Parrish et al., 2009, Aerts et al., 2007, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.10.29, Zeitlinger et al., 2007, Dionne et al., 2006, Ostrin et al., 2006, Yao et al., 2006, Glise et al., 2005, Torroja et al., 2005)
            shf-ov
            (Bateman, 1950)
            Name Synonyms
            Shifted
            (Avanesov and Blair, 2013, Gradilla and Guerrero, 2013, Avanesov et al., 2012, Callejo et al., 2007, Wilson and Chuang, 2006)
            WNT inhibitory factor
            (Banyai and Patthy, 2004)
            shifted
            (Deshpande et al., 2013, Zhai et al., 2010, Kurusu et al., 2008, Ostrin et al., 2006, Wendler et al., 2006, Briscoe and Therond, 2005, Torroja et al., 2005)
            Secondary FlyBase IDs
            • FBgn0029901
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 50 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            References (97)