Isolation and characterization of Df(1)BSC297
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC297 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07849 and PBac{WH}CG3044f02328. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 P{XP}d07849/w1118 PBac{WH}CG3044f02328; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC297 from the segment of P{XP}d07849 to the left of its FRT site and the segment of PBac{WH}CG3044f02328 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC297 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 6C12;6D6. It failed to complement shf2.