Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey produced as a recombinant fusion protein in baculovirus-Sf9 cell system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD