FlyBase Allele Report: Dmel\mew[UAS.cWa]

General Information

Symbol

Dmel\mew^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wehrli

FlyBase ID

FBal0051343

Feature type

allele

Associated gene

Dmel\mew

Associated Insertion(s)

Carried in Construct

P{UAS-mew.W}

Also Known As

UAS-αPS1

Key Links

Transgenic product class

cDNA

(Brabant et al., 1996)

wild_type

(Brabant et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Brabant et al., 1996)

wild_type

(Brabant et al., 1996)

Mutagen

in vitro construct

(Hoang and Chiba, 1998, Brabant et al., 1996, Roote and Zusman, 1996)

Progenitor genotype

Carried in construct

P{UAS-mew.W}

Cytology

Description

UASt regulatory sequences drive expression of mew cDNA.

(Brabant et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mew

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal tracheal branch primordium, with Scer\GAL4^btl.PS

(Boube et al., 2001)

Detailed Description

Statement

Reference

In mew^Scer\UAS.cWa, Scer\GAL4^btl.PS embryos, there is abnormal dorsal branch migration and fusion and occasional dorsal trunk breaks. Many dorsal branches fail to fuse with the dorsal branch from the contralateral hemisegment, or fuse with an inappropriate branch.

(Boube et al., 2001)

Embryos expressing mew^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155 do not show axon defects in the central or peripheral nervous systems.

(Hoang and Chiba, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^NP1301/mew^UAS.cWa is a suppressor of abnormal neuroanatomy phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Scer\GAL4^ap-md544, mew^UAS.cWa, fng^UAS.cKa is a suppressor | partially of visible phenotype of ap^md544/ap^ts78j

(O'Keefe and Thomas, 2001)

NOT Suppressor of

Statement

Reference

mew^UAS.cWa, Scer\GAL4^elav.PU is a non-suppressor of abnormal neuroanatomy phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Yang and Terman, 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^NP1301/mew^UAS.cWa is a suppressor of larval intersegmental nerve branch ISNb of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Scer\GAL4^NP1301/mew^UAS.cWa is a suppressor of larval segmental nerve branch SNa of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301

(Yang and Terman, 2012)

Scer\GAL4^ap-md544, mew^UAS.cWa, fng^UAS.cKa is a suppressor | partially of wing phenotype of ap^md544/ap^ts78j

(O'Keefe and Thomas, 2001)

NOT Suppressor of

Statement

Reference

mew^UAS.cWa, Scer\GAL4^elav.PU is a non-suppressor of larval central nervous system phenotype of PlexA^UAS.Tag:HA, Scer\GAL4^elav.PU

(Yang and Terman, 2012)

Scer\GAL4^ap-md544/mew^UAS.cWa is a non-suppressor of wing phenotype of ap^unspecified

(O'Keefe and Thomas, 2001)

Scer\GAL4⁶⁸⁴/mew^UAS.cWa is a non-suppressor of wing | somatic clone phenotype of if^A7

(Brabant et al., 1996)

Other

Statement

Reference

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has wing vein phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has dorsal row of wing sensilla phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has wing margin phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has alula & macrochaeta | supernumerary phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has sensillum campaniformium | supernumerary & wing vein L3 | ventral phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has ventral row of wing sensilla | increased number phenotype

(O'Keefe and Thomas, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Reduced maintenance phenotype of adult mew^M6/mew^M6, if^k27e/if^k27e double mutant intestinal stem cell clones is partially rescued by expression of mew^UAS.cWa under the control of Scer\GAL4^Act.PU in these clones.

(Lin et al., 2013)

The ISNb and SNa axon guidance defects seen in 14-3-3ε^j2B10/14-3-3ε^NP1301 embryos are significantly suppressed by expression of mew^Scer\UAS.cWa under the control of Scer\GAL4^{14-3-3ε-NP1301}.

(Yang and Terman, 2012)

Expression of mew^Scer\UAS.cWa alone under the control of Scer\GAL4^ap-md544 does not rescue any aspect of the ap^unspecified mutant phenotype. Coexpression of mew^Scer\UAS.cWa and fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 partially rescues the ap^ts78j/ap^md544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristle types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity.

(O'Keefe and Thomas, 2001)

Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa fails to rescue the blistering phenotype of ventral if^A7 clones.

(Brabant et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^btl.PS/mew^UAS.cWa rescues mew^M6

(Boube et al., 2001)

Scer\GAL4^how-24B/mew^UAS.cWa rescues mew⁴⁹⁸

(Li et al., 1998)

mew^UAS.cWa/Scer\GAL4^hs.PB rescues mew⁴⁹⁸

(Li et al., 1998)

Scer\GAL4⁶⁸⁴/mew^UAS.cWa rescues mew⁴⁹⁸

(Brabant et al., 1996)

Scer\GAL4⁶⁸⁴/mew^UAS.cWa rescues mew^M6

(Brabant et al., 1996)

Comments

mew^Scer\UAS.cWa when driven by Scer\GAL4^btl.PS rescues the visceral branch phenotype of mew^M6 embryos.

(Boube et al., 2001)

Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa rescues mew^M6 and mew⁴⁹⁸ dorsal clones completely.

(Brabant et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

M. Wehrli.

(Roote and Zusman, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mew^Scer\UAS.cWa

mew^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Wehrli

(Roote and Zusman, 1996)

Secondary FlyBase IDs

References (9)

Report Sections

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