FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\bsUAS.ORF.GW.Tag:HA
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General Information
Symbol
Dmel\bsUAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0296726
Feature type
allele
Associated gene
Dmel\bs
Associated Insertion(s)
Carried in Construct
M{UAS-bs.ORF.3xHA.GW}
Key Links
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
in vitro construct
(Bischof and FlyORF project members, 2014.6.20)
Progenitor genotype
Carried in construct
M{UAS-bs.ORF.3xHA.GW}
Cytology
Description

UAS regulatory sequences drive expression of a full-length bs open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
bs
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of bsKK108659 under the control of Scer\GAL41151 affects the indirect flight muscles myofibrils (although correctly specified, the muscles show a low polymerized actin content), but not jump muscles, as compared to controls. Although indirect flight muscle myofibril diameter is comparable to controls during mid-pupal stage, it is severely decreased in adults, suggesting myofibrils fail to grow from pupae to adults; however, sarcomere length is normal in both pupae and adults; the myofibril growth defects is rescued by the co-expression of bsUAS.ORF.GW.Tag:HA, with the myofibrils becoming even greater in diameter than those of controls.

      (DeAguero et al., 2019)

      Expression of bsScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with severe wing size and patterning defects.

      Expression of bsScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with weak wing size and patterning defects.

      (Schertel et al., 2015)
      External Data
      Interactions
      Complementation and Rescue Data
      Partially rescues

      bsUAS.ORF.GW.Tag:HA/Scer\GAL41151 partially rescues bsKK108659

      (DeAguero et al., 2019)
      Comments

      The co-expression of bsUAS.ORF.GW.Tag:HA significantly improves the indirect flight muscles defects (actin content and myofibril diameter), but not the flight defects, induced by the expression of bsKK108659 under the control of Scer\GAL41151; and the rescued myofibrils even become greater in diameter than those of controls.

      (DeAguero et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      bsScer\UAS.ORF.GW.T:Ivir\HA1
      bsUAS.ORF.GW.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (5)