Isolation and characterization of Df(2R)BSC780
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC780 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG11406f05598 and P{XP}Mmp1d03549. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG11406f05598/P{XP}Mmp1d03549 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC780 from the segment of PBac{WH}CG11406f05598 to the left of its FRT site and the segment of P{XP}Mmp1d03549 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2R)BSC780 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:20090739 ;20572714 and the cytological breakpoints predicted from these coordinates are 60C2;60D14. Df(2R)BSC780 failed to complement slbo01310, bs3 and Mov34k08003.