FlyBase Allele Report: Dmel\Rpn8[k08003]

General Information

Symbol

Dmel\Rpn8^k08003

Species

D. melanogaster

Name

FlyBase ID

FBal0064357

Feature type

allele

Associated gene

Dmel\Rpn8

Associated Insertion(s)

P{lacW}Rpn8^k08003

Carried in Construct

Also Known As

Mov34^k08003

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Rpn8^k08003

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,403,234..24,403,234 [+]

(Gemmill et al., 2005, Kuo et al., 2005, Watts et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage

(Kuo et al., 2005)

abnormal neuroanatomy | somatic clone

(Watts et al., 2003)

decreased cell number | somatic clone

(Watts et al., 2003)

lethal (with Df(2R)BSC603)

(Kahsai et al., 2016)

lethal (with Df(2R)ED4061)

(Kahsai et al., 2016)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

adult mushroom body | somatic clone

(Watts et al., 2003)

adult mushroom body gamma-lobe | somatic clone

(Watts et al., 2003)

dendritic arborizing neuron | P-stage

(Kuo et al., 2005)

neuroblast | somatic clone

(Watts et al., 2003)

Detailed Description

Statement

Reference

Mov34^k08003 mutants fail to efficiently sever their larval dendrites during metamorphosis, with the retention of approximately 49% of large dendritic branches at 20 hours after pupal formation.

(Kuo et al., 2005)

When homozygous clones are made no gross defects in axon guidance are seen in the mushroom body, however a block on axon pruning during metamorphosis is seen. As a consequence of this phenotype mutant γ neurons also maintain their dorsal projections. A reduction is also seen of cell numbers in neuroblast clones. Mutant clones show a weak age-dependant loss of cell number and axon projections.

(Watts et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Rpn8[+]/Rpn8^k08003 is a non-enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

NOT Suppressor of

Statement

Reference

Rpn8^k08003/Mov34[+] is a non-suppressor of abnormal size phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Rpn8[+]/Rpn8^k08003 is a non-enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

Rpn8[+]/Rpn8^k08003 is a non-enhancer of dendrite | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

NOT Suppressor of

Statement

Reference

Rpn8^k08003/Mov34[+] is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1^HMS00405 under the control of Scer\GAL4^ppk.PG is not significantly modified by combination with a single copy of Rpn8^k08003.

(Herzmann et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rpn8^k16724

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23860

y¹ w^*; P{neoFRT}42D P{lacW}Rpn8^k08003/CyO

Kyoto

111449

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Rpn8^k08003 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: bs⁰³²⁶⁷. Complements: l(2)04012a^04012a. Complements: Nurf-38^k16102.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mov34^k08003

(Ribot et al., 2022, Herzmann et al., 2017, Rumpf et al., 2014, Christensen et al., 2009.5.6, Christensen et al., 2008.9.3)

Rpn8^k08003

l(2)k08003

(Spradling et al., 1999)

l(2)k08003^k08003

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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