FlyBase Allele Report: Dmel\Trc8[UAS.Tag:MYC]

General Information

Symbol

Dmel\Trc8^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0263918

Feature type

allele

Associated gene

Dmel\Trc8

Associated Insertion(s)

Carried in Construct

P{UAS-Trc8.MYC}

Also Known As

Uas-DTrc8

Key Links

Transgenic product class

wild_type

(Gemmill et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gemmill et al., 2002)

Mutagen

in vitro construct

(Gemmill et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Trc8.MYC}

Cytology

Description

UASt drives expression of the Trc8 coding region, beginning 19 bp prior to the AUG, and ending with a Tag:MYC tag and an in-frame stop codon.

(Gemmill et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Trc8

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^ap-md544

(Gemmill et al., 2002)

abnormal size, with Scer\GAL4^Bx-MS1096

(Gemmill et al., 2005, Gemmill et al., 2002)

abnormal size, with Scer\GAL4^en.PU

(Gemmill et al., 2005)

lethal, with Scer\GAL4^Act.PU

(Gemmill et al., 2002)

lethal - all die before end of first instar larval stage, with Scer\GAL4^Act.PU

(Gemmill et al., 2005)

some die during first instar larval stage, with Scer\GAL4^Act.PU

(Gemmill et al., 2005)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Gemmill et al., 2002)

wing, with Scer\GAL4^ap-md544

(Gemmill et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Gemmill et al., 2005, Gemmill et al., 2002)

wing, with Scer\GAL4^en.PU

(Gemmill et al., 2005)

Detailed Description

Statement

Reference

Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} wings are small and deformed. Male wings show a more severe effect than females (probably because of dosage compensation resulting from Scer\GAL4^Bx-MS1096 being located on the X chromosome).

Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} wings are growth inhibited in the posterior compartment, showing an anterior/posterior ratio of 2.36 compared to 1.03 for wild type.

(Gemmill et al., 2005)

Expression of Trc8^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^Act.PU is lethal, while expression using Scer\GAL4^sim.PS or Scer\GAL4^prd.RG1 has no obvious effects.

Expression of Trc8^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^GMR.PU results in loss of pigment and photoreceptors.

Expression of Trc8^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^Bx-MS1096 or Scer\GAL4^ap-md544 results in small, curled wings, compatible with preferential growth inhibition in the dorsal compartment. Using Scer\GAL4^Bx-MS1096, overall wing length and width of the wing is reduced by approximately 20%. These smaller wings result largely from there being fewer cells.

(Gemmill et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, suppressible by CSN5^null/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, suppressible by CSN5[+]/CSN5^quo-3

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, suppressible by Df(3R)hh/+

(Gemmill et al., 2005)

Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has abnormal size phenotype, suppressible | partially by CSN5^null/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has abnormal size phenotype, suppressible | partially by CSN5[+]/CSN5^quo-3

(Gemmill et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, non-suppressible by CSN5^quo-1/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, non-suppressible by CSN5^quo-2/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, non-suppressible by CSN4[+]/CSN4^null

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, non-suppressible by Rpn8^k08003/Mov34[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has abnormal size phenotype, non-suppressible by Rpn11^BG01694/Rpn11[+]

(Gemmill et al., 2005)

Other

Statement

Reference

CSN5^quo-1/CSN5[+], Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has lethal phenotype

(Gemmill et al., 2005)

CSN5^quo-2/CSN5[+], Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has lethal phenotype

(Gemmill et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, suppressible by CSN5^null/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, suppressible by CSN5[+]/CSN5^quo-3

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, suppressible by Df(3R)hh/+

(Gemmill et al., 2005)

Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has wing phenotype, suppressible | partially by CSN5^null/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has wing phenotype, suppressible | partially by CSN5[+]/CSN5^quo-3

(Gemmill et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, non-suppressible by CSN5^quo-1/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, non-suppressible by CSN5^quo-2/CSN5[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, non-suppressible by CSN4[+]/CSN4^null

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, non-suppressible by Rpn8^k08003/Mov34[+]

(Gemmill et al., 2005)

Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC has wing phenotype, non-suppressible by Rpn11^BG01694/Rpn11[+]

(Gemmill et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

CSN5^null/+ or CSN5^quo-3/+ or Df(3R)hh/+ (Df(3R)hh uncovers CSN6) restores normal appearing growth to Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} female wings and substantially increases wing size in male wings.

CSN5^null/+ or CSN5^quo-3/+ partially restores the anterior/posterior size ratio of Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} wings towards wild type.

(Gemmill et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trc8^{Scer\UAS.T:Hsap\MYC}

Trc8^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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