FlyBase Allele Report: Dmel\CSN5[null]

General Information

Symbol

Dmel\CSN5^null

Species

D. melanogaster

Name

FlyBase ID

FBal0141864

Feature type

allele

Associated gene

Dmel\CSN5

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Oron et al., 2002)

Mutagen

P-element activity

(Oron et al., 2002)

Progenitor genotype

Cytology

Description

Imprecise excision of a P-element, resulting in a deletion of CSN5 nucleotides -87 to +1396 (relative to the first nucleotide of the start codon) which removes the entire coding sequence.

(Oron et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:16,470,092..16,471,574 [+]

Comment:

A deletion of sequences from -87 to +1396 relative to the CSN5 translation start, resulting from imprecise P-element excision.

(Oron et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Oron et al., 2002)

abnormal size (with CSN5^quo-3)

(Gemmill et al., 2005)

chemical sensitive | larval stage

(Oron et al., 2002)

female sterile | germline clone | recessive

(Oron et al., 2002)

lethal | recessive

(Gemmill et al., 2005)

lethal - all die before end of larval stage | recessive

(Oron et al., 2002)

lethal - all die before end of pupal stage (with CSN5^quo-1)

(Gemmill et al., 2005)

lethal - all die before end of second instar larval stage (with CSN5^quo-2)

(Gemmill et al., 2005)

partially lethal - majority die (with CSN5^quo-3)

(Gemmill et al., 2005)

some die during larval stage | recessive

(Oron et al., 2002)

some die during pupal stage (with CSN5^quo-1)

(Gemmill et al., 2005)

some die during second instar larval stage (with CSN5^quo-2)

(Gemmill et al., 2005)

visible | adult stage | somatic clone

(Suisse et al., 2017)

visible | somatic clone

(Mummery-Widmer et al., 2009, Oren-Giladi et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult flies carrying CSN5^null mutant clones in the wings display extra wing veins.

(Suisse et al., 2017)

Homozygous clones in the thorax result in hair cell duplication phenotypes.

(Mummery-Widmer et al., 2009)

About half of CSN5^quo-3/CSN5^null animals emerge as adults. These escapers are overall smaller than wild type and have droopy wings.

(Gemmill et al., 2005)

Homozygotes do not pupate, remaining in a prolonged third larval instar. Mutant larvae do not show moulting defects. Mutant third instar larvae develop melanotic capsules in their hemolymph, which are first seen floating freely in the hemocoel 4 days after the second moult and increase in frequency until by the late third instar over 80% of the mutant larvae contain these capsules. Embryos are not recovered from females containing homozygous germline clones. Oogenesis is arrested at stage 5-6 in these females. Homozygous larvae are sensitive to methyl methanesulfonate compared to controls.

(Oron et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CSN5^null/CSN5[+] is a suppressor of abnormal size phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

CSN5^null/CSN5[+] is a suppressor | partially of abnormal size phenotype of Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

CSN5^null/CSN5[+] is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

CSN5^null/CSN5[+] is a suppressor | partially of wing phenotype of Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

CSN5^null/+ restores normal appearing growth to Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} female wings and substantially increases wing size in male wings.

CSN5^null/+ partially restores the anterior/posterior size ratio of Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} wings towards wild type.

(Gemmill et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CSN5^null1.3

(Gemmill et al., 2005)

CSN5^null

(Suisse et al., 2017, Kim et al., 2010, Mummery-Widmer et al., 2009, Chan et al., 2008, Chan et al., 2008, Rencus-Lazar et al., 2008, Wu et al., 2005)

csn5^null

(Oren-Giladi et al., 2008, Oron et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (10)

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