FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\mspo
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General Information
Symbol
Dmel\mspo
Species
D. melanogaster
Name
M-spondin
Annotation Symbol
CG10145
Feature Type
protein_coding_gene
FlyBase ID
FBgn0020269
Gene Model Status
Current
Stock Availability
79 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
51B9-51C1
Sequence location
Recombination map
2-72
RefSeq locus
NT_033778 REGION:14671396..14713622
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of myoblast fusion
inferred from mutant phenotype
(Bataillé et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in cell adhesion
inferred from biological aspect of ancestor with PANTHER:PTN002259349
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in extracellular matrix
inferred from direct assay
(Umemiya et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in extracellular matrix
inferred from biological aspect of ancestor with PANTHER:PTN002259349
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SPONDINS -
Spondin family proteins are secreted extracellular matrix glycoproteins that share thrombospondin type I repeats and F-Spondin domains. (Adapted from FBrf0096489.)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mspo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7JY80)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mspo-RA
FBtr0087477
NM_166063
2574
601
mspo-RB
FBtr0345427
NM_001299498
3131
601
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mspo-PA
FBpp0086606
65.4
601
7.99
Q7JY80
NP_725407
AAF58219
mspo-PB
FBpp0311552
65.4
601
7.99
Q7JY80
NP_001286427
AHN56225
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

601 aa isoforms: mspo-PA, mspo-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mspo using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.68

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
larval imaginal tissue | restricted
(Grubbs et al., 2013)
embryonic stage 4 -- 6
anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hindgut anlage in statu nascendi
(Fisher et al., 2012)
embryonic stage 7 -- 8
hindgut anlage
(Fisher et al., 2012)
embryonic stage 13 -- 16
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)
embryonic brain
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
larval circulatory system
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval pericardial cell
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
ventral sensory complex primordium
(Fisher et al., 2012)
embryonic stage 15
muscle cell of ventral acute muscle 2 | intense
(Bataillé et al., 2010)
muscle cell of A1-7 dorsal transverse muscle 1 | intense
(Bataillé et al., 2010)
muscle cell of dorsal acute muscle 1 | faint
(Bataillé et al., 2010)
muscle cell of ventral transverse muscle 1 | faint
(Bataillé et al., 2010)
Additional Descriptive Data

At embryonic stage 15, mspo transcript is expressed at high level in the A1-7 dorsal transverse muscle 1, at a median level in ventral acute muscle 2, a low level in dorsal acute muscle 1 and ventral transverse muscle 1 and are not detectable in the segment border muscle.

(Bataillé et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in extracellular matrix
inferred from direct assay
(Umemiya et al., 1997)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mspo in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mspo
Transgenic constructs containing regulatory region of mspo
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)Exel6284
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\SPON2
11 of 14
Yes
Yes
Hsap\SPON1
3 of 14
No
No
Hsap\ADGRB2
1 of 14
No
No
Hsap\RSPO3
1 of 14
No
No
Hsap\THSD7A
1 of 14
No
No
Hsap\THSD7B
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Spon2
12 of 14
Yes
Yes
Rnor\Spon1
2 of 14
No
No
Rnor\Rspo2
1 of 14
No
No
Rnor\Rspo3
1 of 14
No
No
Rnor\Thsd7a
1 of 14
No
No
Rnor\Thsd7b
1 of 14
No
No
Mus musculus (laboratory mouse) (6)
Mmus\Spon2
10 of 14
Yes
Yes
Mmus\Spon1
3 of 14
No
No
Mmus\Rspo2
1 of 14
No
No
Mmus\Rspo3
1 of 14
No
No
Mmus\Thsd7a
1 of 14
No
Yes
Mmus\Thsd7b
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (10)
Xtro\spon2
9 of 13
Yes
Yes
Xtro\adamtsl5
1 of 13
No
No
Xtro\adgrb1
1 of 13
No
Yes
Xtro\LOC100492479
1 of 13
No
No
Xtro\myof.1
1 of 13
No
No
Xtro\rspo2
1 of 13
No
Yes
Xtro\sbspon
1 of 13
No
No
Xtro\spon1
1 of 13
No
No
Xtro\thsd7a
1 of 13
No
Yes
Xtro\thsd7b
1 of 13
No
Yes
Danio rerio (Zebrafish) (9)
Drer\spon2a
10 of 14
Yes
Yes
Drer\spon2b
10 of 14
Yes
Yes
Drer\spon1a
3 of 14
No
No
Drer\spon1b
3 of 14
No
No
Drer\rspo3
1 of 14
No
No
Drer\si:dkey-178e17.3
1 of 14
No
No
Drer\thsd7aa
1 of 14
No
Yes
Drer\thsd7ab
1 of 14
No
Yes
Drer\thsd7ba
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\spon-1
3 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (5)
Agam\AgaP_AGAP012534
4 of 12
Yes
Yes
Agam\AgaP_AGAP011765
3 of 12
No
No
Agam\AgaP_AGAP012307
3 of 12
No
No
Agam\AgaP_AGAP010795
2 of 12
No
No
Agam\AgaP_AGAP012813
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:mspo. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (5)
CG17739
5 of 13
CG30203
5 of 13
CG30046
4 of 13
fat-spondin
4 of 13
vex
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SPON2; spondin 2
    11 of 14
    605918
        SPON1; spondin 1
        3 of 14
        604989
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            mspo
            suppressible
            Mp20
            (Bataillé et al., 2010)
            mspo
            suppressible
            Pax
            (Bataillé et al., 2010)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-72
            Cytogenetic map
            51B9-51C1
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            51B9-51C1
            Limits computationally determined from genome sequence between P{lacW}ttvk03617&P{EP}ttvEP765 and P{lacW}Rpn6k00103
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            51C-51C
            (Umemiya, 1997.4.17)
            Experimentally Determined Recombination Data
            Location

            2-72

            (FlyBase, 2016)

            2-76.3

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (79)
            Genomic Clones (43)
            cDNA Clones (43)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                Cloning and characterisation of a secretory protein localised at the MASs, encoded by the mspo gene.

                (Umemiya et al., 1997)
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (7)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 43 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (74)