FlyBase Allele Report: Dmel\Mlp84B[GD6893]

General Information

Symbol

Dmel\Mlp84B^GD6893

Species

D. melanogaster

Name

FlyBase ID

FBal0207631

Feature type

allele

Associated gene

Dmel\Mlp84B

Associated Insertion(s)

Carried in Construct

P{GD6893}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6893}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6893

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013, Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Mlp84B^GD6893 under the control of Scer\GAL4^Mef2.PR does not affect the size of newly-hatched (0-12 hour) larvae, compared to controls, and their muscles have prominent Z-lines and no obvious defect in muscle attachments.

(Clark and Kadrmas, 2013)

Flies expressing Mlp84B^GD6893 under the control of either one of Scer\GAL4^dpp.blk1, Scer\GAL4^en-e16E or Scer\GAL4^en-e16E display normal planar cell polarity in the eye and the wing.

(Weber et al., 2012)

Adults expressing Mlp84B^GD6893 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6893

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Mlp84B^GD6893, Scer\GAL4^Mef2.PR is an enhancer of some die during larval stage phenotype of Actn^GD1354, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Mlp84B^GD6893, Scer\GAL4^Mef2.PR is an enhancer of decreased body size | larval stage phenotype of Actn^GD1354, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Mlp84B^GD6893, Scer\GAL4^Mef2.PR is an enhancer of visible | larval stage phenotype of Actn^GD1354, Scer\GAL4^Mef2.PR

(Clark and Kadrmas, 2013)

Mlp84B^GD6893/Scer\GAL4^Mef2.PR is an enhancer of increased mortality during development phenotype of Actn^CC01961

(Clark and Kadrmas, 2013)

Mlp84B^GD6893, Scer\GAL4^hs.2sev is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

NOT Enhancer of

Statement

Reference

Mlp84B^GD6893, Scer\GAL4^hs.2sev is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Weber et al., 2012)

NOT Suppressor of

Statement

Reference

Mlp84B^GD6893, Scer\GAL4^hs.2sev is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Weber et al., 2012)

Other

Statement

Reference

Actn^CC01961, Mlp84B^GD6893, Scer\GAL4^Mef2.PR has lethal - all die before end of pupal stage phenotype

(Clark and Kadrmas, 2013)

Dcr-2^UAS.cDa, Mlp84B^GD6893, Scer\GAL4^hs.2sev has abnormal planar polarity phenotype

(Weber et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

Mlp84B^GD6893, Scer\GAL4^hs.2sev is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

NOT Enhancer of

Statement

Reference

Mlp84B^GD6893, Scer\GAL4^hs.2sev is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Weber et al., 2012)

NOT Suppressor of

Statement

Reference

Mlp84B^GD6893/Scer\GAL4^Mef2.PR is a non-suppressor of muscle attachment site | larval stage phenotype of Actn^GD1354

(Clark and Kadrmas, 2013)

Mlp84B^GD6893, Scer\GAL4^hs.2sev is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Weber et al., 2012)

Other

Statement

Reference

Actn^GD1354, Mlp84B^GD6893, Scer\GAL4^Mef2.PR has embryonic/larval hypodermal muscle cell | larval stage phenotype

(Clark and Kadrmas, 2013)

Actn^GD1354, Mlp84B^GD6893, Scer\GAL4^Mef2.PR has Z disc | larval stage phenotype

(Clark and Kadrmas, 2013)

Dcr-2^UAS.cDa, Mlp84B^GD6893, Scer\GAL4^hs.2sev has ommatidium phenotype

(Weber et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mlp84B^GD6893 and Actn^GD1354 under the control of Scer\GAL4^Mef2.PR, in larvae, causes severe disruption of muscle structure, with some wispy muscle fibers, and Z-lines that are hard to discern; these phenotypes are not seen in controls or in either single knock-down.

(Clark and Kadrmas, 2013)

Co-expression of Mlp84B^GD6893 and Dcr-2^Scer\UAS.cDa using Scer\GAL4^hs.2sev results in planar cell polarity (PCP)-abnormalities in ommatidia. Mlp84B^GD6893 also induces some loss of ommatidia.

(Weber et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v18593

w¹¹¹⁸; P{GD6893}v18593

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6893

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mlp84B^GD6893

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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