FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wts, jub
FlyBase ID
FBig0000101808
Interaction Network
Interactions Browser links
wts network
jub network
Reported Interactions
FBrf0221057-2.coIP.WB
Reference
Reddy and Irvine, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
jub 

Jub

bait

FLAG tag

wts 

Wts

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The jub-wts interaction increases upon activation of MAPK signaling by cotransfection of Ras85D[V12], phl, Dsor1 and rl.

(Reddy and Irvine, 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Reddy and Irvine, 2013)
FBrf0225521-2.coIP.WB
Reference
Rauskolb et al., 2014
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
jub 

Jub

bait

GFP tag

wts 

Wts

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The jub-wts interaction increases when sqh (which elevates cytoskeletal tension) is expressed.

(Rauskolb et al., 2014)

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Rauskolb et al., 2014)
FBrf0241561-2.coIP.WB
Reference
Alégot et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wts 

Wts

bait

V5 tag

jub 

Jub

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Alégot et al., 2019)

Positive control.

(Alégot et al., 2019)
FBrf0254236-2.coIP.WB
Reference
Rauskolb et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wts 

wts-Cat

bait

V5 tag

jub 

Jub

prey

FLAG tag

jub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
jub 
Lim2 domain
necessary binding region
aa 693-718
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

jub Lim2 domain required in all constructs tested.

(Rauskolb et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)