FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
α-Cat, jub
FlyBase ID
FBig0000104847
Interaction Network
Interactions Browser links
α-Cat network
jub network
Reported Interactions
FBrf0225521-1.coIP.WB
Reference
Rauskolb et al., 2014
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
jub 

Jub

bait

GFP tag

α-Cat 

α-catenin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The jub-α-Cat interaction increases when sqh (which elevates cytoskeletal tension) is expressed.

(Rauskolb et al., 2014)

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Rauskolb et al., 2014)
FBrf0241561-1.coIP.WB
Reference
Alégot et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

α-cat

bait

V5 tag

α-Cat 
jub 

Jub

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Cat 
N2 domain
sufficient binding region
aa 148-272

coordinates relative to α-Cat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Alégot et al., 2019)
FBrf0254236-1.coIP.WB
Reference
Rauskolb et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

α-Cat

bait

V5 tag

α-Cat 
jub 

Jub

prey

FLAG tag

jub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Cat 
N2 domain
sufficient binding region
aa 152-272
jub 
prelim plus Lim2 and Lim3 domains
mutation increasing interaction
aa 1-505 and 571-718
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Multiple regions of jub interacted with the N2 domain of α-Cat. The prelim plus Lim2 and Lim3 domains of jub had a 780% stronger interaction compared to wild type.

(Rauskolb et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)