FlyBase Allele Report: Dmel\zfh1[UAS.cPa]

General Information

Symbol

Dmel\zfh1^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Postigo

FlyBase ID

FBal0140829

Feature type

allele

Associated gene

Dmel\zfh1

Associated Insertion(s)

Carried in Construct

P{UAS-zfh1.P}

Also Known As

UAS-zfh1, UAS-zfh-1

Key Links

Transgenic product class

wild_type

(Postigo, 2002.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Postigo, 2002.12)

Mutagen

in vitro construct

(Postigo, 2002.12)

Progenitor genotype

Carried in construct

P{UAS-zfh1.P}

Cytology

Description

Expression of a full length zfh1 coding sequence is governed by UAS regulatory sequences.

(Postigo, 2002.12)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

zfh1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

ameliorates central nervous system cancer

modeled by N^CA.UAS.cUa

(Magadi et al., 2020)

modeled by E(spl)mγ-HLH^UAS.Tag:HA, E(spl)m8-HLH^UAS.Tag:HA

(Magadi et al., 2020)

modeled by E(spl)m8-HLH^UAS.Tag:HA, dpn^UAS.Tag:HA

(Magadi et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^A3.eya

(Leatherman and DiNardo, 2008)

viable, with Scer\GAL4^A3.eya, Scer\GAL80^ts.αTub84B

(Leatherman and DiNardo, 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of zfh1^Scer\UAS.cPa in somatic cells under the control of Scer\GAL4^A3.eya (restricted to adult stages using Scer\GAL80^ts.αTub84B) results in testes that are filled with undifferentiated cells. Small early stage germ cells are intermingled with excess somatic cells. The excess germ cells are primarily found together in pairs, although individual cells and larger groupings of spermatogonia are also present. The pairs of germ cells seen typically share a spherical fusome and, as in wild type, they are surrounded by somatic extensions. They cycle as single cells in locations far removed from the hub. Occasionally excess somatic cells accumulate without accompanying germ cells. Dividing somatic cells are often seen, even far from the hub.

(Leatherman and DiNardo, 2008)

Embryos expressing zfh1^Scer\UAS.cPa under the control of Scer\GAL4^how-24B show a reduction in size of the DO1 muscles and the DO1 and DO2 muscles are more loosely associated compared to wild type. In addition, the number and size of the DA1 muscles is reduced.

(Johnson et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^eve.RN2/zfh1^UAS.cPa is a suppressor | partially of abnormal neuroanatomy phenotype of grn^7L/Df(3R)dsx3

(Garces and Thor, 2006)

Scer\GAL4^eve.RN2/zfh1^UAS.cPa is a suppressor | partially of abnormal neuroanatomy phenotype of Df(3R)dsx3/grn^SPJ9

(Garces and Thor, 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^eve.RN2/zfh1^UAS.cPa is a suppressor | partially of larval DA1 motor neuron phenotype of grn^7L/Df(3R)dsx3

(Garces and Thor, 2006)

Scer\GAL4^eve.RN2/zfh1^UAS.cPa is a suppressor | partially of larval RP2 motor neuron phenotype of grn^7L/Df(3R)dsx3

(Garces and Thor, 2006)

Scer\GAL4^eve.RN2/zfh1^UAS.cPa is a suppressor | partially of larval intersegmental nerve phenotype of Df(3R)dsx3/grn^SPJ9

(Garces and Thor, 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/zfh1^UAS.cPa is a non-suppressor of cyst progenitor cell | somatic clone | adult stage phenotype of chinmo¹

(Flaherty et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of zfh1^Scer\UAS.cPa in the aCC/RP2 neurons, under the control of Scer\GAL4^eve.RN2 can only partially rescue the grn^7L/Df(3R)dsx3 or grn^SPJ9/Df(3R)dsx3 motoneuron phenotype, with muscle 1/9 innervation increasing to 34% compared with the more severe (approximately 12%) grn^7L/Df(3R)dsx3 or grn^SPJ9/Df(3R)dsx3 mutant phenotype.

(Garces and Thor, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^how-24B/zfh1^UAS.cPa rescues zfh1²

(Frandsen et al., 2008)

Comments

Expression of zfh1^Scer\UAS.cPa under the control of the pan-mesodermal driver Scer\GAL4^how-24B in zfh1² mutants rescues the lymph gland phenotype.

(Frandsen et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6879

w¹¹¹⁸; P{UAS-zfh1.P}2B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

zfh1^Scer\UAS.cPa

zfh1^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Postigo

(Postigo, 2002.12)

Secondary FlyBase IDs

References (20)

Report Sections

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