FlyBase Gene Report: Dmel\grau

General Information

Symbol

Dmel\grau

Species

D. melanogaster

Name

grauzone

Annotation Symbol

CG33133

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001133

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

grauzone (grau) encodes a transcription factor with the Cdc20 activator of the Anaphase Promoting Complex (APC) encoded by cort as key target. Absence of grau function results in the cort phenotype of female sterility, meiosis II arrest, and egg activation defects. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

57B9-57B9

Sequence location

2R:21,009,764..21,011,921 [-]

Recombination map

2-92

RefSeq locus

NT_033778 REGION:21009764..21011921

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cis-regulatory region sequence-specific DNA binding

inferred from direct assay

(Chen et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001144693

(GO Reference Genome Project, 2011-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR039970

(InterPro Project Members, 2004-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR012934

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in cellularization

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1989)

involved_in egg activation

inferred from mutant phenotype

(Tadros et al., 2003)

involved_in female meiotic nuclear division

inferred from mutant phenotype

(Chen et al., 2000)

involved_in pole cell formation

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1989)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Chen et al., 2000)

involved_in regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Harms et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in homologous chromosome segregation

traceable author statement

(Johnstone and Lasko, 2001)

involved_in regulation of transcription by RNA polymerase II

traceable author statement

(Johnstone and Lasko, 2001)

inferred from biological aspect of ancestor with PANTHER:PTN001144693

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Chen et al., 2000)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001144693

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR012934

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Protein Function (UniProtKB)

Transcription factor essential for the completion of meiosis in oocytes. Grauzone binds to the promoter region of cort via the zf-AD domain and activates cort expression in ovaries.

(UniProt, Q9U405)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\grau for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9U405)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

1PZW

Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

grau-RA

FBtr0071548

NM_176242

1894

570

Additional Transcript Data and Comments

Reported size (kB)

1.876 (sequence analysis)

(Chen et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

grau-PA

FBpp0071477

66.0

570

7.57

Q9U405

NP_788422

AAF46653

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

570 (aa); 66 (kD predicted)

(Chen et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer; at the zf-AD.

(UniProt, Q9U405)

Domain

The N-terminal zf-AD is an atypical treble-clef like zinc binding domain which enables dimerization, and appears not to have any DNA-binding ability. DNA binding is achieved through the zinc fingers at the C-terminus.

(UniProt, Q9U405)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1PZW

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9U405

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\grau using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

grau_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.10

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Chen et al., 2000)

adult stage | female

(Chen et al., 2000)

adult stage | male

(Chen et al., 2000)

adult stage & oogenesis

ovary

(Chen et al., 2000)

Additional Descriptive Data

grau transcripts are detected in ovaries, embryos, larvae, pupae, adult males, and adult females with ovaries removed.

(Chen et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

germarium

(Chen et al., 2000)

nurse cell

(Chen et al., 2000)

follicle cell

(Chen et al., 2000)

oogenesis stage S1 -- S10B & adult stage | female

ovary

(Chen et al., 2000)

Additional Descriptive Data

The distribution of grau protein was studied with the use of a grau-GFP fusion protein. grau protein is observed in ovaries in the nuclei of nurse cells and follicle cells. It is observed from the germarium stage up to oocyte stage 10B.

(Chen et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Chen et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\grau in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\grau

View exonic expression by tissue for Dmel\grau

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001133

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45871/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001133

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001133

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001133

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33133

Flygut - An atlas of the Drosophila adult midgut

FBgn0001133

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\grau

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of grau

Transgenic constructs containing regulatory region of grau

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)C4

(Chen et al., 2000)

Df(2R)E2

(Chen et al., 2000)

Df(2R)Pu-D17

(Chen et al., 2000, Page and Orr-Weaver, 1996, Schupbach and Wieschaus, 1991, Schupbach and Wieschaus, 1989)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect | recessive

partially lethal | maternal effect

partially lethal - majority die, with Scer\GAL4^pnr-MD237

grau^GD4077

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

grau^GD4077

viable, with Scer\GAL4^Mef2.PR

grau^GD4077

viable, with Scer\GAL4^tin.CΔ4

grau^GD4077

Sterility

Allele

female sterile

female sterile | recessive

Other Phenotypes

Allele

abnormal meiotic cell cycle

abnormal meiotic cell cycle | maternal effect (with grau^QQ36)

grau^RM61

abnormal meiotic cell cycle | maternal effect (with grau^RM61)

grau^QQ36

abnormal meiotic cell cycle | recessive

grau^QF31

some die during embryonic stage | recessive | maternal effect | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

grau^GD4077

wild-type

Phenotype manifest in

Allele

primordial germ cell

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (177)

Hsap\FEZF2

1 of 14

Yes

Hsap\GTF3A

1 of 14

Yes

Hsap\IKZF1

1 of 14

Yes

Hsap\LOC344065

1 of 14

Yes

Hsap\MECOM

1 of 14

Yes

Hsap\MYNN

1 of 14

Yes

Hsap\MZF1

1 of 14

Yes

Hsap\PATZ1

1 of 14

Yes

Hsap\PRDM5

1 of 14

Yes

Hsap\PRDM9

1 of 14

Yes

Hsap\REPIN1

1 of 14

Yes

Hsap\ZBTB6

1 of 14

Yes

Hsap\ZBTB11

1 of 14

Yes

Hsap\ZBTB24

1 of 14

Yes

Hsap\ZBTB37

1 of 14

Yes

Hsap\ZBTB39

1 of 14

Yes

Hsap\ZBTB49

1 of 14

Yes

Hsap\ZFP1

1 of 14

Yes

Hsap\ZFP2

1 of 14

Yes

Hsap\ZFP62

1 of 14

Yes

Hsap\ZFP91

1 of 14

Yes

Hsap\ZFP92

1 of 14

Yes

Hsap\ZFX

1 of 14

Yes

Hsap\ZFY

1 of 14

Yes

Hsap\ZIK1

1 of 14

Yes

Hsap\ZIM3

1 of 14

Yes

Hsap\ZKSCAN8

1 of 14

Yes

Hsap\ZNF2

1 of 14

Yes

Hsap\ZNF8

1 of 14

Yes

Hsap\ZNF16

1 of 14

Yes

Hsap\ZNF41

1 of 14

Yes

Hsap\ZNF45

1 of 14

Yes

Hsap\ZNF57

1 of 14

Yes

Hsap\ZNF74

1 of 14

Yes

Hsap\ZNF75D

1 of 14

Yes

Hsap\ZNF77

1 of 14

Yes

Hsap\ZNF79

1 of 14

Yes

Hsap\ZNF83

1 of 14

Yes

Hsap\ZNF84

1 of 14

Yes

Hsap\ZNF85

1 of 14

Yes

Hsap\ZNF90

1 of 14

Yes

Hsap\ZNF91

1 of 14

Yes

Hsap\ZNF92

1 of 14

Yes

Hsap\ZNF93

1 of 14

Yes

Hsap\ZNF98

1 of 14

Yes

Hsap\ZNF99

1 of 14

Yes

Hsap\ZNF100

1 of 14

Yes

Hsap\ZNF112

1 of 14

Yes

Hsap\ZNF132

1 of 14

Yes

Hsap\ZNF133

1 of 14

Yes

Hsap\ZNF135

1 of 14

Yes

Hsap\ZNF141

1 of 14

Yes

Hsap\ZNF146

1 of 14

Yes

Hsap\ZNF154

1 of 14

Yes

Hsap\ZNF157

1 of 14

Yes

Hsap\ZNF160

1 of 14

Yes

Hsap\ZNF169

1 of 14

Yes

Hsap\ZNF175

1 of 14

Yes

Hsap\ZNF180

1 of 14

Yes

Hsap\ZNF182

1 of 14

Yes

Hsap\ZNF184

1 of 14

Yes

Hsap\ZNF202

1 of 14

Yes

Hsap\ZNF208

1 of 14

Yes

Hsap\ZNF225

1 of 14

Yes

Hsap\ZNF226

1 of 14

Yes

Hsap\ZNF227

1 of 14

Yes

Hsap\ZNF229

1 of 14

Yes

Hsap\ZNF234

1 of 14

Yes

Hsap\ZNF235

1 of 14

Yes

Hsap\ZNF236

1 of 14

Yes

Hsap\ZNF254

1 of 14

Yes

Hsap\ZNF264

1 of 14

Yes

Hsap\ZNF268

1 of 14

Yes

Hsap\ZNF273

1 of 14

Yes

Hsap\ZNF282

1 of 14

Yes

Hsap\ZNF287

1 of 14

Yes

Hsap\ZNF311

1 of 14

Yes

Hsap\ZNF316

1 of 14

Yes

Hsap\ZNF317

1 of 14

Yes

Hsap\ZNF319

1 of 14

Yes

Hsap\ZNF333

1 of 14

Yes

Hsap\ZNF335

1 of 14

Yes

Hsap\ZNF337

1 of 14

Yes

Hsap\ZNF341

1 of 14

Yes

Hsap\ZNF345

1 of 14

Yes

Hsap\ZNF350

1 of 14

Yes

Hsap\ZNF354C

1 of 14

Yes

Hsap\ZNF358

1 of 14

Yes

Hsap\ZNF382

1 of 14

Yes

Hsap\ZNF398

1 of 14

Yes

Hsap\ZNF416

1 of 14

Yes

Hsap\ZNF419

1 of 14

Yes

Hsap\ZNF420

1 of 14

Yes

Hsap\ZNF423

1 of 14

Yes

Hsap\ZNF425

1 of 14

Yes

Hsap\ZNF430

1 of 14

Yes

Hsap\ZNF431

1 of 14

Yes

Hsap\ZNF442

1 of 14

Yes

Hsap\ZNF443

1 of 14

Yes

Hsap\ZNF454

1 of 14

Yes

Hsap\ZNF471

1 of 14

Yes

Hsap\ZNF473

1 of 14

Yes

Hsap\ZNF480

1 of 14

Yes

Hsap\ZNF484

1 of 14

Yes

Hsap\ZNF486

1 of 14

Yes

Hsap\ZNF492

1 of 14

Yes

Hsap\ZNF497

1 of 14

Yes

Hsap\ZNF501

1 of 14

Yes

Hsap\ZNF502

1 of 14

Yes

Hsap\ZNF510

1 of 14

Yes

Hsap\ZNF521

1 of 14

Yes

Hsap\ZNF526

1 of 14

Yes

Hsap\ZNF527

1 of 14

Yes

Hsap\ZNF540

1 of 14

Yes

Hsap\ZNF549

1 of 14

Yes

Hsap\ZNF551

1 of 14

Yes

Hsap\ZNF555

1 of 14

Yes

Hsap\ZNF557

1 of 14

Yes

Hsap\ZNF558

1 of 14

Yes

Hsap\ZNF567

1 of 14

Yes

Hsap\ZNF568

1 of 14

Yes

Hsap\ZNF569

1 of 14

Yes

Hsap\ZNF570

1 of 14

Yes

Hsap\ZNF573

1 of 14

Yes

Hsap\ZNF577

1 of 14

Yes

Hsap\ZNF582

1 of 14

Yes

Hsap\ZNF583

1 of 14

Yes

Hsap\ZNF587

1 of 14

Yes

Hsap\ZNF595

1 of 14

Yes

Hsap\ZNF605

1 of 14

Yes

Hsap\ZNF614

1 of 14

Yes

Hsap\ZNF616

1 of 14

Yes

Hsap\ZNF619

1 of 14

Yes

Hsap\ZNF624

1 of 14

Yes

Hsap\ZNF626

1 of 14

Yes

Hsap\ZNF629

1 of 14

Yes

Hsap\ZNF639

1 of 14

Yes

Hsap\ZNF646

1 of 14

Yes

Hsap\ZNF648

1 of 14

Yes

Hsap\ZNF658

1 of 14

Yes

Hsap\ZNF665

1 of 14

Yes

Hsap\ZNF675

1 of 14

Yes

Hsap\ZNF679

1 of 14

Yes

Hsap\ZNF684

1 of 14

Yes

Hsap\ZNF699

1 of 14

Yes

Hsap\ZNF710

1 of 14

Yes

Hsap\ZNF714

1 of 14

Yes

Hsap\ZNF718

1 of 14

Yes

Hsap\ZNF721

1 of 14

Yes

Hsap\ZNF723

1 of 14

Yes

Hsap\ZNF726

1 of 14

Yes

Hsap\ZNF727

1 of 14

Yes

Hsap\ZNF728

1 of 14

Yes

Hsap\ZNF729

1 of 14

Yes

Hsap\ZNF732

1 of 14

Yes

Hsap\ZNF737

1 of 14

Yes

Hsap\ZNF749

1 of 14

Yes

Hsap\ZNF775

1 of 14

Yes

Hsap\ZNF778

1 of 14

Yes

Hsap\ZNF782

1 of 14

Yes

Hsap\ZNF786

1 of 14

Yes

Hsap\ZNF792

1 of 14

Yes

Hsap\ZNF808

1 of 14

Yes

Hsap\ZNF816

1 of 14

Yes

Hsap\ZNF835

1 of 14

Yes

Hsap\ZNF836

1 of 14

Yes

Hsap\ZNF837

1 of 14

Yes

Hsap\ZNF845

1 of 14

Yes

Hsap\ZNF846

1 of 14

Yes

Hsap\ZNF850

1 of 14

Yes

Hsap\ZNF865

1 of 14

Yes

Hsap\ZNF879

1 of 14

Yes

Hsap\ZNF880

1 of 14

Yes

Hsap\ZSCAN2

1 of 14

Yes

Hsap\ZSCAN12

1 of 14

Yes

Hsap\ZSCAN21

1 of 14

Yes

Hsap\ZSCAN30

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Zbtb24

1 of 14

Yes

Rnor\Zfp26

1 of 14

Yes

Rnor\Zfp54

1 of 14

Yes

Rnor\Zfp62

1 of 14

Yes

Rnor\Zfp74

1 of 14

Yes

Rnor\Zfp78

1 of 14

Yes

Rnor\Zfp112

1 of 14

Yes

Rnor\Zfp180

1 of 14

Yes

Rnor\Zfp334

1 of 14

Yes

Rnor\Zfp354c

1 of 14

Yes

Rnor\Zfp423

1 of 14

Yes

Rnor\Zfp521

1 of 14

Yes

Rnor\Zfp846

1 of 14

Yes

Rnor\Zik1

1 of 14

Yes

Rnor\Zim1

1 of 14

Yes

Rnor\Zscan2

1 of 14

Yes

Mus musculus (laboratory mouse) (27)

Mmus\2610021A01Rik

1 of 14

Yes

Mmus\BC024063

1 of 14

Yes

Mmus\Gm5141

1 of 14

Yes

Mmus\Gm14295

1 of 14

Yes

Mmus\Gm14412

1 of 14

Yes

Mmus\Prdm5

1 of 14

Yes

Mmus\Zfp26

1 of 14

Yes

Mmus\Zfp58

1 of 14

Yes

Mmus\Zfp62

1 of 14

Yes

Mmus\Zfp74

1 of 14

Yes

Mmus\Zfp78

1 of 14

Yes

Mmus\Zfp112

1 of 14

Yes

Mmus\Zfp142

1 of 14

Yes

Mmus\Zfp180

1 of 14

Yes

Mmus\Zfp184

1 of 14

Yes

Mmus\Zfp334

1 of 14

Yes

Mmus\Zfp354c

1 of 14

Yes

Mmus\Zfp423

1 of 14

Yes

Mmus\Zfp454

1 of 14

Yes

Mmus\Zfp457

1 of 14

Yes

Mmus\Zfp521

1 of 14

Yes

Mmus\Zfp558

1 of 14

Yes

Mmus\Zfp560

1 of 14

Yes

Mmus\Zfp846

1 of 14

Yes

Mmus\Zfp930

1 of 14

Yes

Mmus\Zik1

1 of 14

Yes

Mmus\Zscan2

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (52)

Xtro\cxcl9

1 of 13

Yes

Xtro\fezf1

1 of 13

Yes

Xtro\has3

1 of 13

Yes

Xtro\LOC496795

1 of 13

Yes

Xtro\LOC100135213

1 of 13

Yes

Xtro\LOC100485062

1 of 13

Yes

Xtro\LOC100487439

1 of 13

Yes

Xtro\LOC100487628

1 of 13

Yes

Xtro\LOC100488006

1 of 13

Yes

Xtro\LOC100489584

1 of 13

Yes

Xtro\LOC100489897

1 of 13

Yes

Xtro\LOC100498654

1 of 13

Yes

Xtro\LOC101731232

1 of 13

Yes

Xtro\LOC101735222

1 of 13

Yes

Xtro\LOC116406443

1 of 13

Yes

Xtro\LOC116406487

1 of 13

Yes

Xtro\LOC116411645

1 of 13

Yes

Xtro\MGC146893

1 of 13

Yes

Xtro\mynn

1 of 13

Yes

Xtro\ovol2

1 of 13

Yes

Xtro\prdm15

1 of 13

Yes

Xtro\sall4

1 of 13

Yes

Xtro\siah3

1 of 13

Yes

Xtro\smc1b

1 of 13

Yes

Xtro\snai1

1 of 13

Yes

Xtro\tmem35b

1 of 13

Yes

Xtro\vps28

1 of 13

Yes

Xtro\XB5795368

1 of 13

Yes

Xtro\xlcgf26.1l

1 of 13

Yes

Xtro\zbtb1

1 of 13

Yes

Xtro\zbtb11

1 of 13

Yes

Xtro\zbtb37

1 of 13

Yes

Xtro\zbtb40

1 of 13

Yes

Xtro\zbtb47

1 of 13

Yes

Xtro\zfp91

1 of 13

Yes

Xtro\zfx

1 of 13

Yes

Xtro\znf84

1 of 13

Yes

Xtro\znf142

1 of 13

Yes

Xtro\znf177

1 of 13

Yes

Xtro\znf276

1 of 13

Yes

Xtro\znf319

1 of 13

Yes

Xtro\znf408

1 of 13

Yes

Xtro\znf420

1 of 13

Yes

Xtro\znf423

1 of 13

Yes

Xtro\znf521

1 of 13

Yes

Xtro\znf526

1 of 13

Yes

Xtro\znf527

1 of 13

Yes

Xtro\znf574

1 of 13

Yes

Xtro\znf585a

1 of 13

Yes

Xtro\znf639

1 of 13

Yes

Xtro\znf665

1 of 13

Yes

Xtro\znf865

1 of 13

Yes

Danio rerio (Zebrafish) (93)

Drer\si:ch1073-127d16.1

2 of 14

Yes

Drer\LOC557537

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC101882402

1 of 14

Yes

Drer\LOC101884305

1 of 14

Drer\LOC101884367

1 of 14

Drer\LOC101885996

1 of 14

Yes

Drer\LOC101886047

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC108183691

1 of 14

Drer\LOC108183935

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:cabz01032454.3

1 of 14

Drer\si:cabz01071909.2

1 of 14

Yes

Drer\si:ch73-266f23.4

1 of 14

Yes

Drer\si:ch73-367f21.4

1 of 14

Drer\si:ch73-367f21.6

1 of 14

Drer\si:ch211-202h22.10

1 of 14

Yes

Drer\si:ch211-207i20.2

1 of 14

Yes

Drer\si:ch211-216l23.1

1 of 14

Drer\si:ch211-222k6.1

1 of 14

Drer\si:ch211-229l10.11

1 of 14

Drer\si:dkey-14d8.1

1 of 14

Yes

Drer\si:dkey-33c14.6

1 of 14

Drer\si:dkey-57k17.1

1 of 14

Drer\si:dkey-66i24.8

1 of 14

Yes

Drer\si:dkey-77f5.4

1 of 14

Yes

Drer\si:dkey-182i3.8

1 of 14

Drer\si:dkey-226l10.6

1 of 14

Yes

Drer\si:dkey-253d23.2

1 of 14

Drer\si:dkey-253d23.9

1 of 14

Yes

Drer\si:dkey-262g12.3

1 of 14

Yes

Drer\si:dkeyp-104f11.6

1 of 14

Yes

Drer\si:rp71-1h20.5

1 of 14

Yes

Drer\zbtb48

1 of 14

Drer\zgc:101562

1 of 14

Yes

Drer\zgc:113119

1 of 14

Yes

Drer\zgc:113135

1 of 14

Yes

Drer\zgc:113294

1 of 14

Yes

Drer\zgc:113295

1 of 14

Drer\zgc:153116

1 of 14

Yes

Drer\zgc:162972

1 of 14

Drer\zgc:171220

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\znf142

1 of 14

Yes

Drer\znf408

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\znf569l

1 of 14

Drer\znf971

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\ZK686.5

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (55)

Agam\AgaP_AGAP005938

9 of 12

Yes

8 of 12

7 of 12

7 of 12

7 of 12

6 of 12

Yes

Agam\AgaP_AGAP005473

6 of 12

Agam\AgaP_AGAP005479

6 of 12

Yes

Agam\AgaP_AGAP005485

6 of 12

Yes

Agam\AgaP_AGAP004575

4 of 12

Yes

Agam\AgaP_AGAP005482

4 of 12

Yes

Agam\AgaP_AGAP005348

3 of 12

Agam\AgaP_AGAP007513

3 of 12

Yes

Agam\AgaP_AGAP007514

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP007915

1 of 12

Agam\AgaP_AGAP007947

1 of 12

Agam\AgaP_AGAP008971

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP011544

1 of 12

Agam\AgaP_AGAP011759

1 of 12

Agam\AgaP_AGAP012768

1 of 12

Arabidopsis thaliana (thale-cress) (24)

Atha\AT1G02030

1 of 13

Yes

Atha\AT1G26610

1 of 13

Yes

Atha\AT2G28200

1 of 13

Yes

Atha\AT2G28710

1 of 13

Yes

Atha\AT3G10470

1 of 13

Yes

Atha\AT3G46070

1 of 13

Yes

Atha\AT3G46080

1 of 13

Yes

Atha\AT3G49930

1 of 13

Yes

Atha\AT3G53600

1 of 13

Yes

Atha\AT3G60580

1 of 13

Yes

Atha\AT4G16610

1 of 13

Yes

Atha\AT5G03510

1 of 13

Yes

Atha\AT5G04390

1 of 13

Yes

Atha\DAZ1

1 of 13

Yes

Atha\DAZ2

1 of 13

Yes

Atha\RHL41

1 of 13

Yes

Atha\STZ

1 of 13

Yes

Atha\YY1

1 of 13

Yes

Atha\ZAT6

1 of 13

Yes

Atha\ZAT7

1 of 13

Yes

Atha\ZAT11

1 of 13

Yes

Atha\ZF1

1 of 13

Yes

Atha\ZF2

1 of 13

Yes

Atha\ZF3

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ZAP1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:grau. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

8 of 13

7 of 13

7 of 13

4 of 13

3 of 13

3 of 13

3 of 13

az2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

peb

1 of 13

prg

1 of 13

Saft

1 of 13

wek

1 of 13

zfh1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

grau

pull down, molecular weight estimation by staining, two hybrid

(Bonchuk et al., 2021)

grau - CG11695

two hybrid

(Bonchuk et al., 2021)

grau - CG15073

two hybrid, pull down, molecular weight estimation by staining

(Bonchuk et al., 2021)

grau - CG2712

pull down, molecular weight estimation by staining

(Bonchuk et al., 2021)

grau - Hr78

two hybrid array

(Shokri et al., 2019)

grau - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

grau - abd-A

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

grau - eEF1α1

pull down, Identification by mass spectrometry, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Zolotarev et al., 2016)

grau - p53

pull down, autoradiography

(Lunardi et al., 2010)

grau - retn

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

grau

enhanceable

cort

(Chen et al., 2000)

grau

suppressible

cort

(Harms et al., 2000)

grau

suppressible

mtrm

(Whitfield et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer; at the zf-AD.

(UniProt, Q9U405 )

Linkouts

BioGRID - A database of protein and genetic interactions.

69943

DroID - A comprehensive database of gene and protein interactions.

FBgn0001133

MIST (genetic) - An integrated Molecular Interaction Database

45871

MIST (protein-protein) - An integrated Molecular Interaction Database

45871

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-92

Cytogenetic map

57B9-57B9

Sequence location

2R:21,009,764..21,011,921 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57B9-57B9

Limits computationally determined from genome sequence between P{PZ}l(2)07806⁰⁷⁸⁰⁶ and P{EP}grau^EP688&P{PZ}shg¹⁰⁴⁶⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

57B5-57B14

(Chen, 1999.11.23)

Experimentally Determined Recombination Data

Location

2-92

(FlyBase, 2016)

2-99.5

(Comeron et al., 2012)

2-97

(Schupbach and Wieschaus, 1989)

Left of (cM)

P{PZ}ND-B14.7⁰³⁰⁵⁰

(Chen et al., 2000)

(Chen et al., 1998)

Right of (cM)

P{PZ}CG34115⁰⁷⁸⁰⁶

(Chen et al., 2000)

(Chen et al., 1998)

Notes

Mapping uses male recombination stimulated by P\T^Δ2-3.

(Chen et al., 1998)

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

17191

w¹¹¹⁸; P{EP}grau^EP688

53251

y¹ v¹; P{TRiP.GLC01810}attP2

41940

y¹ v¹; P{TRiP.HMS02337}attP40

81262

y¹ w^*; PBac{grau-GFP.FPTB}VK00033

5018

cn¹ grau^QQ36 bw¹/CyO, l(2)DTS513¹

FlyORF

F003292

M{UAS-grau.ORF-CC}ZH-21F

F000452

M{UAS-grau.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

3282R-1

P{NIG.3282R}1

HMS02337

y¹ v¹; P{TRiP.HMS02337}attP40/CyO

VDRC

v104196

P{KK104206}VIE-260B

v47600

w¹¹¹⁸; P{GD4077}v47600

v341395

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00457}attP40/CyO-GFP

More stocks available...

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (150)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH04731

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001133

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001133

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The activation of cort transcription is the primary role of grau protein during oogenesis.

(Harms et al., 2000)

cort and grau genes are necessary for bcd protein expression.

(Lieberfarb et al., 1996)

grau and cort are involved in the developmental signalling of the completion of meiosis in females.

(Page and Orr-Weaver, 1996)

Mutations in grau result in a maternal effect phenotype with defects during syncytial blastoderm formation.

(Schupbach and Wieschaus, 1989)

Relationship to Other Genes

Source for database merge of

Source for merge of: grau CG3282

(Bayraktaroglu, 2000.8.7)

Additional comments

Annotation CG3282 split into CG33133 (grau) and CG30291 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Name Synonyms

Grauzone

(Nie et al., 2009, Chung et al., 2007)

grauzone

(Weil et al., 2008, Pesin and Orr-Weaver, 2007, Mukai et al., 2006, Chung et al., 2002)

Secondary FlyBase IDs

FBgn0034571
FBgn0053133

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

45871

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9U405

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9U405

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9U405

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001133

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45871/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001133

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001133

FlyMine - An integrated database for Drosophila genomics

FBgn0001133

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33133

MARRVEL_MODEL - MARRVEL (model organism gene)

45871

PDB - An information portal to biological macromolecular structures

1PZW

Linkouts

BioGRID - A database of protein and genetic interactions.

69943

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001133

DroID - A comprehensive database of gene and protein interactions.

FBgn0001133

DRSC - Results frm RNAi screens

FBgn0001133

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

grau_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001133

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001133

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33133

Flygut - An atlas of the Drosophila adult midgut

FBgn0001133

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034895

MIST (genetic) - An integrated Molecular Interaction Database

45871

MIST (protein-protein) - An integrated Molecular Interaction Database

45871

References (84)

The following external sites may use different assemblies or annotations than FlyBase.

1.10

Report Sections