Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
2.0 (northern blot)
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lbl using the Feature Mapper tool.
lbe transcript appears earlier, and is more abundant, than the lbl transcript. Both transcripts are expressed in the anal plate primordium, with lbl expression starting at stage 10-11. At stage 10-11, expression is also detected in a cluster of cells corresponding to the heart primordium. During embryonic stages 10-13, lbe and lbl are both expressed in the dorsal ectoderm of abdominal segments 1-7, and to a lesser extent, in the ventral ectoderm. At stage 15, lbl and lbe transcripts persist in the dorsal, but not in the ven ral, epidermis. Later in embryogenesis, lbl and lbe transcripts are restricted to clusters of cells in the central and peripheral nervous systems, and to the segment border muscles.
Using RT-PCR, lbl transcript is detected in embryogenesis 2 hours after lbe, and persists through embryogenesis. lbl transcript is detected in stage 13 embryos, in the dorsal and ventral epidermis in a segmentally repeated pattern.
Using RT-PCR, lbl transcript is detected in 8-20 hr embryos, in third instar larvae, and in pupae. In-situ hybridization to stage 13-17 embryos reveals that lbl is expressed in parts of the developing central nervous system, including cells of the ventral nerve cord. Strongest expression is seen during early ventral cord condensation.
GBrowse - Visual display of RNA-Seq signalsView Dmel\lbl in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The specific epidermal expression patterns of lbe and lbl and their regulatory interactions with wg are analysed. Activity of lbe and lbl in the epidermis is regulated by the segment polarity gene network and depends on wg signalling. lbe and lbl are required in the dorsal epidermis and for anal plate development.
lbe and lbl are involved in the specification of a segmental subset of heart precursors during the early phase of cardiogenesis, and are required for maintaining wg activity during the late phase of cardiogenesis.