Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
Stop-codon suppression (UGA) postulated; FBrf0234051.
Gene model reviewed during 6.25
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\otp using the Feature Mapper tool.
The earliest otp expression is visible in the the proctodeum at embryonic stage 9. otp expression in the nervous system begins at stage 10 and expression in the hindgut, anal pads, ventral nerve cord, and brain is observed at stage 14. Expression is also observed in the rectum, at a lower leve than in the other parts of the hindgut. In the nervous system, there is a clear border with a higher level of otp expression in the five most anterior segments of the ventral nerve cord and a lower level in the posterior segments of the ventral nerve cord. Expression in the nervous system is specific to the larger 2.9kb alternative transcript that starts with exon 1 (e.g. otp-RE). Expression in the hindgut primordium, embryonic hindgut and anal pads is specific to the smaller 2.3kb transcripts (e.g. otp-RC) that initiates at exon 3.
otp protein expression is first observed in the proctodeum at embryonic stage 10. By stage 14, it is observed in the hindgut, anal pads, ventral nerve cord, and brain. In the ventral nerve cord, expression is only observed up to segment A2 and not in more posteriorly-located segments. In the embryonic brain, expression was seen in three discrete expression domains per hemisphere, two (P1 and P2) in the protocerebrum and one (D1) in the deutocerebrum. The expression domains consist of 10 (P1), 3 (P2) and 5 (D1) neurons. otp protein is also expressed in the larval and adult ileum and rectum but not in the pylorus.
GBrowse - Visual display of RNA-Seq signalsView Dmel\otp in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Annotations CG10036, CG2965 merged as CG10036 in release 3 of the genome annotation, April 2002.
One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site.