Subject: homeodomains and wombats
Dear Rachel,
Prof. Rob Saint asked me to reply to your email regarding the Flybase
information
I have independently cloned a homeobox gene, called bk24, identical in amino
acid sequence within the homeodomain to W26. Like W26, the gene maps at 57B
and is expressed in the hindgut.
The reference for this paper is Kalionis, B. and O'Farrell, P.H. (1993)
Mechanisms of Development 43, 57-70
In this paper you will also find the homeodomain sequences of wom (bk50) and
emu (bk36) neither of them are related to W26. The chromosome locations,
expression patterns and cDNA sequences of wom and emu are unpublished.
My clone bk24, which is described in the above paper is identical at the DNA
and amino acid sequence level to W26 in the homeodomain. I checked this with
Walter Gehring several years ago. I confirmed that bk24 locates to 57B.
Please let me know if you need further details.
Regards,
Dr. Bill Kalionis