mus308, mutagen-sensitive 308, Pol θ
DGC data (RH44346) contradicts with the Genscan predition and introduces premature stop codon, thus was disregarded.
Gene model reviewed during 5.48
There is only one protein coding transcript and one polypeptide associated with this gene
2059 (aa); 229 (kD predicted)
In adult males, cleaved to produce a 100 kDa form.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolQ using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\PolQ in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
During end-joining repair of DNA double-strand breaks, mus308 plays a crucial role in Lig4-independent alternative end joining. Mutant analysis suggests that mus308 may be involved in two processes associated with alternative end joining: first, annealing at short, complementary DNA sequences and second, DNA synthesis that creates small insertions at break repair sites.
mus308 is important for both interstrand crosslink repair and end-joining repair of DNA double-strand breaks.
mus308 is required to process ethyl nitrosourea (ENU) induced lesions, and may play a role in post replication bypass of O-alkylpyrimidines, probably mediated by recombination, which serves to increase the time available for error-free repair of these lesions.
Gene is involved in pre-replication repair of lesions produced by DNA cross-link agents.
The carboxy terminal domain shares over 55% sequence similarity with the polymerase domains of prokaryotic DNA polymerase I-like enzymes. This is the first member of this family of DNA polymerases to be identified in a eukaryotic organism. mus308 is closely related to a gene in C.elegans suggesting this novel polypeptide may play an evolutionarily conserved role in the repair of DNA damage in eukaryotic organisms.
The sex linked recessive lethal test has been used to measure the mutability of mus308 mutants to several mutagens. Results suggest mus308 is not defective in a repair pathway specific for cross-links but is rather involved in a step of a more general post-replication process responsible for the removal of non-excised adducts.
"3-51 +/- 1" was stated as revision.
The mus308 locus is unique among mutagen sensitive mutations in that mutants are hypersensitive to DNA cross-linking agents but not to monofunctional alkylating agents.
mus308 gene is required for resistance to DNA cross-linking agents, normal female fertility and chromosome stability.