FlyBase Allele Report: Dmel\PolQ[D2]

General Information

Symbol

Dmel\PolQ^D2

Species

D. melanogaster

Name

FlyBase ID

FBal0012656

Feature type

allele

Associated gene

Dmel\PolQ

Associated Insertion(s)

Carried in Construct

Also Known As

mus308^D2

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Boyd et al., 1981)

Progenitor genotype

Cytology

Description

No mutations have been found in the coding sequence.

(Chan et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing

(Leonhardt et al., 1993)

Detailed Description

Statement

Reference

mus308^D2/Df(3R)Exel6166 larvae are unable to survive exposure to 0.005% mechlorethamine (nitrogen mustard).

mus308^D2/Df(3R)Exel6166 animals are able to survive exposures of as high as 4000rads of ionising radiation.

Studies of excision and repair events of P{hsw^a} in mus308^D2/mus308^Sz29 males indicate that DNA repair synthesis during synthesis-dependent strand annealing (SDSA) is unaffected in the mutants but that the mutants are defective in end-joining repair of DNA double-strand breaks. There is a substantial increase in the percentage of deletion-associated repair events isolated from mus308^D2 mutant males relative to those isolated from wild-type males.

(Chan et al., 2010)

mus308^D2 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32^oC or 38^oC.

(Wei and Rong, 2007)

A mus308^D2 maternal background does not significantly alter the frequency of sex linked recessive lethal mutations induced by acrolein. The hypermutability index for mus308^D2 is 0.52.

(Barros et al., 1994)

Excision repair capacity is normal and post-replication repair capacity is deficient.

(Ferro and Eeken, 1993)

A partial deficiency in the excision repair of pyrimidine dimers originally observed in this mutant is now attributed to the presence of a secondary phr mutation, phr^mus308-D2.

(Harris and Boyd, 1993)

Adult survivors of sub-lethal nitrogen mustard treatments exhibit an incompletely penetrant held up wing phenotype, with reduced flight ability. Viability of untreated embryos is reduced, and chromatin organization in inviable embryos is abnormal.

(Leonhardt et al., 1993)

Mutations exhibit a partial deficiency in the capacity to excise UV-induced pyrimidine dimers. In addition cells show defective post-replicative repair of UV damage.

(Todo and Ryo, 1992)

Survival of homozygous larvae is hypersensitive to nitrogen mustard, diepoxybutane and cis-platinum, but not to methyl methanesulfonate. Larval survival is also sensitive to UV treatment and trimethylpsoralen feeding when applied together.

(Boyd et al., 1990)

Flies are moderately sensitive to X rays.

(Oliveri et al., 1990)

Flies exposed to nitrogen mustard show an increase in mitochondrial abnormalities compared to wild-type.

(Sakaguchi et al., 1990)

Cells derived from homozygous embryos synthesise DNA at a reduced rate both from an undamaged template and after UV irradiation compared to wild-type cells. Homozygous larval brain ganglia have a reduced ability to synthesise DNA from an undamaged template compared to wild-type.

(Boyd and Shaw, 1982)

Sensitive to 0.002% HN2. Not sensitive to 0.125% methyl methanesulfonate.

(Boyd et al., 1981)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

PolQ^D5/PolQ^D2, spn-A^093A/spn-A³ has partially lethal phenotype

(Chan et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hsw^a} in spn-A³ mus308^D2/spn-A³ mus308^ZIII-2003 and spn-A³ mus308^D2/spn-A³ mus308^D2 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show a substantial increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

spn-A³ mus308^D2/spn-A³ mus308^ZIII-2003 double mutant males in which excision of P{hsw^a} is occurring show morphological defects such as abdominal cuticle mispatterning.

(Chan et al., 2010)

mus308^D2/mus308^D4 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

(McKee et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

249

PolQ^D2

Notes on Origin

Discoverer

Boyd.

Comments

In spite of the hypersensitivity to DNA cross-linking agents, the initial incision step of DNA cross-link repair appears normal in mus308^D2 homozygotes.

(Boyd et al., 1990)

Studies of Nuc3 activity in mus308^D2 mutants support the hypothesis that the mus308 locus is not the structural gene for Nuc3, but is involved in modifying the pI of the Nuc3 enzyme.

(Sakaguchi et al., 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

308^D2

(McKee et al., 2000)

(Chan et al., 2010)

DNApol-θ^D2

PolQ^D2

mus308^D2

(Beagan et al., 2017, White and Lambowitz, 2012, Díaz-Valdés et al., 2010, Wei and Rong, 2007, Pang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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