FlyBase Allele Report: Dmel\spn-A[3]

General Information

Symbol

Dmel\spn-A³

Species

D. melanogaster

Name

FlyBase ID

FBal0016031

Feature type

allele

Associated gene

Dmel\spn-A

Associated Insertion(s)

Carried in Construct

Also Known As

spn-A⁰⁵⁷, spnA⁰⁵⁷, Rad51⁰⁵⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Staeva-Vieira et al., 2003, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: A205V.

(Staeva-Vieira et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:30,055,263..30,055,263 [-]

Nucleotide change:

C30055263T

Amino acid change:

A205V | spn-A-PA; A148V | spn-A-PB

Reported amino acid change:

A205V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Staeva-Vieira et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

radiation sensitive

(Chan et al., 2010, Staeva-Vieira et al., 2003)

radiation sensitive (with spn-A^093A)

(McVey et al., 2004)

viable (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

visible

(Li et al., 2014)

Phenotype Manifest In

egg | dorsal

(Gonzalez-Reyes et al., 1997)

egg chamber

(Gonzalez-Reyes et al., 1997)

egg chorion

(Li et al., 2014)

germline cyst

(Huynh and St. Johnston, 2000)

karyosome

(Gonzalez-Reyes et al., 1997)

nurse cell

(Gonzalez-Reyes et al., 1997)

oocyte

(Gonzalez-Reyes et al., 1997)

synaptonemal complex

(Huynh and St. Johnston, 2000)

Detailed Description

Statement

Reference

Larval exposure to hydroxyurea does not affect spn-A³/spn-A³ adult survival rates.

(Bolterstein et al., 2014)

Mutants show sensitivity to ionising radiation compared to controls, with almost no survival of the mutants after exposure to 750rads ionising radiation.

(Chan et al., 2010)

spn-A³/spn-A^093A males in which excision of an insertion of P{hsw^a} is induced during development are completely viable.

(LaRocque et al., 2007)

spn-A³/spn-A^093A third instar larvae show increased sensitivity to high doses of gamma irradiation compared to controls.

(McVey et al., 2004)

The complete copia element in w^a.hs has an LTR at each end. Following excision of P{hsw^a}, repair by SDSA (synthesis-dependent strand annealing) can lead to excision of this copia element due to annealing at these LTRs, resulting in red eyed progeny. Incomplete SDSA, where joining has occurred independently of annealing of homologous ends, can delete parts of the w ORF in w^a.hs resulting in yellow eyed progeny. The frequency of red eyed progeny (and therefore of complete SDSA) following excision of P{hsw^a}sd^wa using H{PΔ2-3}HoP2.1 is decreased from 6% to zero in a spn-A³/spn-A^093A background, but the frequency of yellow eyed progeny (and therefore of incomplete SDSA) is increased from 10% to 17%. This background does not significantly affect the proportion of hemizygous lethal sd deletions resulting from this excision, but does decrease the proportion of excisions resulting non-lethal deletions (from 7% to 1.7%).

(McVey et al., 2004)

In contrast to wild-type ovaries, where the synaptonemal complex (SC) is always restricted to the oocyte by region 2b, spn-A³ mutant females show a significant delay in the process, with cysts with more than 1 cell in synapsis in region 3 of the germarium and in stage 1 and stage 2 of the vitellarium.

(Huynh and St. Johnston, 2000)

Transheterozygous eggs with spn-A² exhibit either a strong or weak ventralised phenotype: eggs are longer than wild type and are completely symmetric along the DV axis or the eggs display fused dorsal appendages. Germline clones in egg chambers are composed of wild type follicle cells, mutant nurse cells, misplaced oocyte, oocytes that give rise to ventralised eggs and oocytes that lack a karyosome.

(Gonzalez-Reyes et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spn-A³ has radiation sensitive phenotype, enhanceable by PolQ^D5

(Chan et al., 2010)

spn-A^093A/spn-A³ has radiation sensitive phenotype, enhanceable by DNAlig4¹⁶⁹

(McVey et al., 2004)

Suppressed by

Statement

Reference

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, suppressible by PolQ^+tBa

(Beagan et al., 2017)

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, suppressible by PolQ^ATPase-dead

(Beagan et al., 2017)

spn-A^093A/spn-A³ has visible phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

NOT suppressed by

Statement

Reference

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, non-suppressible by PolQ^pol-dead

(Beagan et al., 2017)

Enhancer of

Statement

Reference

spn-A^093A/spn-A³ is an enhancer of chemical sensitive phenotype of mus302^D1/mus302^Z1882

(Carvajal-Garcia et al., 2020)

spn-A³/spn-A³ is an enhancer of short lived | chemical conditional phenotype of WRNexo^Δ

(Bolterstein et al., 2014)

NOT Enhancer of

Statement

Reference

spn-A³ is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

spn-A³/PolD3^L2 is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

spn-A³ is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

NOT Suppressor of

Statement

Reference

spn-A³ is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

spn-A³ is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

spn-A³/PolD3^L2 is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

Other

Statement

Reference

PolD3^L2, spn-A³ has viable phenotype

(Kocak et al., 2019)

Pif1¹⁶⁷, spn-A³ has viable phenotype

(Kocak et al., 2019)

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype

(Beagan et al., 2017)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority die phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, Gen^Z5997, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority live phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

PolQ^D5/PolQ^D2, spn-A^093A/spn-A³ has partially lethal phenotype

(Chan et al., 2010)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal | pupal stage phenotype

(Andersen et al., 2009)

DNAlig4¹⁶⁹, spn-A^093A/spn-A³ has viable phenotype

(McVey et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-A³ has phenotype, enhanceable by spn-D²

(Huynh and St. Johnston, 2000)

spn-A³ has synaptonemal complex phenotype, enhanceable by spn-D²

(Huynh and St. Johnston, 2000)

Suppressed by

Statement

Reference

spn-A^093A/spn-A³ has egg chorion phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

Enhancer of

Statement

Reference

spn-A³ is an enhancer of phenotype of spn-D²

(Huynh and St. Johnston, 2000)

spn-A³ is an enhancer of synaptonemal complex phenotype of spn-D²

(Huynh and St. Johnston, 2000)

spn-A³ is an enhancer of oocyte phenotype of spn-D²

(Gonzalez-Reyes et al., 1997)

Other

Statement

Reference

mus301⁰⁹⁴, spn-A³ has oocyte phenotype

(Díaz de la Loza et al., 2017, Gonzalez-Reyes et al., 1997)

spn-A³, spn-D² has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

spn-A³, spn-E¹ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The hypersensitivity of Df(3R)mus308^Δ/Df(3R)mus308^Δ;spn-A³/spn-A^093A double mutant third instar larvae to ionizing radiation is rescued by combination with either the mus308^+tBa or the mus308^ATPase-dead transgene but not by mus308^pol-dead.

Df(3R)mus308^Δ/Df(3R)mus308^Δ;spn-A³/spn-A^093A mutants show a significant reduction in the frequency of end joining repair events in a P{hsw[a]}-excision repair assay, which can be rescued with mus308^+tBa and mus308^ATPase-dead but not mus308^pol-dead.

(Beagan et al., 2017)

spn-A³/spn-A³ enhances hydroxyurea sensitivity (decreases adult survival) in WRNexo^Δ/WRNexo^Δ mutants.

(Bolterstein et al., 2014)

eIF-1A^BH167 dominantly suppresses the eggshell patterning defects in spn-A³/spn-A^093A mutants.

(Li et al., 2014)

spn-A³ mus308^D5 double mutant animals are extremely sensitive to ionising radiation, with almost no survival of the mutants after exposure to 125rads ionising radiation.

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hsw^a} in spn-A³ mus308^D2/spn-A³ mus308^ZIII-2003, spn-A³ mus308^D2/spn-A³ mus308^D2 and spn-A^093A mus308^D5/spn-A³ mus308^ZIII-2003 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show a substantial increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

spn-A³ mus308^D2/spn-A³ mus308^ZIII-2003 double mutant males in which excision of P{hsw^a} is occurring show morphological defects such as abdominal cuticle mispatterning.

(Chan et al., 2010)

The spn-A³/spn-A^093A transheterozygous condition does not suppress the lethality of mus312^D1/mus312^Z1973, mus309^D2/mus309^N1 double mutants.

(Andersen et al., 2009)

The lethality seen in mei-41^29D males in which excision of an insertion of P{hsw^a} is induced during development is completely suppressed by spn-A³/spn-A^093A.

(LaRocque et al., 2007)

At intermediate doses (400-500 rad) of gamma irradiation, Lig4¹⁶⁹ spn-A³/spn-A^093A double mutant larvae show increased sensitivity to irradiation compared to spn-A³/spn-A^093A single mutants.

(McVey et al., 2004)

spn-A³/spn-A^093A suppresses the increase deletions resulting from the H{PΔ2-3}HoP2.1 driven of excision of P{hsw^a}sd^wa due to a mus309^D2/mus309^D3 background.

(McVey et al., 2004)

The phenotype of a delay in the restriction of the synaptonemal complex seen in the female germline of spn-D² or spn-A³ single mutants is enhanced in spn-D² spn-A¹ double mutants.

(Huynh and St. Johnston, 2000)

Double mutant stage 5-6 egg chambers with mus301⁰⁹⁴, spn-D² or spn-E¹ exhibit a two-oocyte phenotype: the second pro-oocyte develops as an oocyte rather than a nurse cell. The double mutants delay but do not block the decision between the two pro-oocytes as one of the two cells always becomes a nurse cell eventually.

(Gonzalez-Reyes et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong mutation.

(Gonzalez-Reyes et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

057

(Staeva-Vieira et al., 2003)

Rad51⁰⁵⁷

(Bolterstein et al., 2014)

rad51⁰⁵⁷

(Kocak et al., 2019)

spn-A⁰⁵⁷

(Khodaverdian et al., 2024, Thomas et al., 2022, Carvajal-Garcia et al., 2020, Fasulo et al., 2020, Beagan et al., 2017, Díaz de la Loza et al., 2017, Do et al., 2014, Kuo et al., 2014, Lafave et al., 2014, Chan et al., 2010, Andersen et al., 2009, LaRocque et al., 2007, McVey et al., 2004, Findley et al., 2003, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)

spn-A³

spn-A⁵⁷

(McVey et al., 2004)

spnA⁰⁵⁷

(Goldstein et al., 2025, Yu and McVey, 2010, McVey et al., 2004, Huynh and St. Johnston, 2000)

spnA³

(Li et al., 2014)

spnA⁵⁷

(Klovstad et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (26)

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