FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PolQD5
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General Information
Symbol
Dmel\PolQD5
Species
D. melanogaster
Name
FlyBase ID
FBal0012659
Feature type
allele
Associated gene
Dmel\PolQ
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Boyd et al., 1981)
Progenitor genotype
Cytology
Description

Amino acid replacement: P781L.

(Chan et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:12,709,745..12,709,745 [-]
Nucleotide change:

C12709745T

Amino acid change:

P781L | PolQ-PA

Reported amino acid change:

P781L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Chan et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mus308D5/Df(3R)Exel6166 larvae are unable to survive exposure to 0.005% mechlorethamine (nitrogen mustard).

      mus308D5/Df(3R)Exel6166 animals are able to survive exposures of as high as 4000rads of ionising radiation.

      Studies of excision and repair events of P{hswa} in mus308D5/mus308Sz29 males indicate that DNA repair synthesis during synthesis-dependent strand annealing (SDSA) is unaffected in the mutants but that the mutants are defective in end-joining repair of DNA double-strand breaks. There is a substantial increase in the percentage of deletion-associated repair events isolated from mus308D5 mutant males relative to those isolated from wild-type males.

      (Chan et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      PolQD5 is an enhancer of radiation sensitive phenotype of spn-A3

      (Chan et al., 2010)
      Other
      Statement
      Reference

      PolQD5/PolQD2, spn-A093A/spn-A3 has partially lethal phenotype

      (Chan et al., 2010)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      spn-A3 mus308D5 double mutant animals are extremely sensitive to ionising radiation, with almost no survival of the mutants after exposure to 125rads ionising radiation.

      There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hswa} in spn-A093A mus308D5/spn-A3 mus308ZIII-2003 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show a substantial increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

      There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hswa} in spn-A093A mus308D5/spn-A093A mus308ZIII-3294 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show an increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

      (Chan et al., 2010)

      mus308D1/mus308D5 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

      (McKee et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Boyd.

      Separable from: a second unidentified mutagen-sensitive mutant allele.

      (Boyd et al., 1981)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      308D5
      (McKee et al., 2000)
      D5
      (Chan et al., 2010)
      DNApol-θD5
      PolQD5
      mus308D5
      Name Synonyms
      Secondary FlyBase IDs
        References (4)