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General Information
Symbol
Dmel\BlmN1
Species
D. melanogaster
Name
FlyBase ID
FBal0218249
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mus309N1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EPgy2}mus309EY03745 insertion, resulting in a 2480bp deletion that removes mus309 sequences, including the start codon and part of the helicase domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval exposure to hydroxyurea decreases (dose-dependently) BlmN1/BlmN1 adult survival rates.

BlmN1/BlmD2 animals are hyper-sensitive to the cross-linking agent HN2, the mono-adduct generator MMS, and ionizing radiation.

Spontaneous mitotic crossovers are elevated in BlmN1/BlmD2 males.

BlmN1/BlmD2 animals show a severely decreased ability to complete 'synthesis-dependent strand annealing' repair of a DNA gap.

BlmN1/BlmN1 flies are short lived and exhibit a significant increase in the frequency of tumor formation in the gut at 35 days-old, with tumors partially or fully occluding the intestinal lumen, and germline tumors consisting of masses of proliferating cells that are morphologically similar to stem cells, as compared to wild type. No significant increase of tumor frequency is observed in 60 day old adults, as compared to wild type.

Embryos derived from homozygous mus309N1/mus309D3 and mus309N1/mus309D2 females have an extremely low hatch rate.

Only 40% of the embryos derived from mus309N1/mus309D2 females have cellularised by 4-6 hours after egg laying, and none have gastrulated by this time (in contrast to embryos derived from wild-type females where 99% have cellularised and 96% have gastrulated by this time).

Homozygous larvae are hypersensitive to gamma radiation, showing reduced survival to adulthood after irradiation with doses of radiation that do not have a large effect on the survival of wild-type larvae.

Studies of excision and repair events of P{hswa} in mus309N1/mus309D2 males indicate that synthesis-dependent strand annealing (SDSA) is severely compromised in these animals.

mus309N1/mus309D2 males show elevated rates of mitotic crossing over compared to control males.

Apoptosis in the wing disc is increased 3- to 4-fold in mus309N1 mutants compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference

BlmN1 has neoplasia phenotype, non-enhanceable by DNAlig4169/DNAlig4169

Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

BlmN1/BlmN1 is a non-enhancer of short lived | chemical conditional phenotype of WRNexoΔ

Other
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

BlmN1 has testis | adult stage phenotype, non-enhanceable by DNAlig4169/DNAlig4169

BlmN1 has ovary | adult stage phenotype, non-enhanceable by DNAlig4169/DNAlig4169

BlmN1 has adult gut phenotype, non-enhanceable by DNAlig4169/DNAlig4169

Suppressed by
NOT suppressed by
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

BlmN1/BlmN1 WRNexoΔ/WRNexoΔ double mutants have similar hydroxyurea sensitivity to either single mutant.

The rate of spontaneous mitotic crossovers in BlmN1/BlmD2 Fancm0693/Df(3R)ED6058 males is not significantly different from that in Fancm0693/Df(3R)ED6058 males, but is significantly lower than in BlmN1/BlmD2 males.

mus309N1/mus309D2 slx1F93I and mus309N1/mus309D2 Df(3R)HKK1, Dp(3;3)HKK2 double mutant animals die as early pupae, larvae lack imaginal discs and larval neuroblasts are frequently polyploid.

mus309N1/mus309D2 mus312D1/mus312Z1973 double mutant larvae have small brains, lack imaginal discs and have a reduced number of salivary gland imaginal ring cells. The nuclei of the remaining salivary gland imaginal ring cells appear larger than normal. Mitotic neuroblasts in these larvae show extreme genome instability; no mitotic nuclei with completely intact chromosomes are detected and approximately one-third show polyploidy.

Mitotic neuroblasts in mus81Nhe ; mus309N1/mus309D2 double mutant larvae show an increased frequency of broken chromosomes compared to wild type.

The addition of spn-Aunspecified significantly decreases the number of chromosome breaks seen in mitotic neuroblasts of mus81Nhe ; mus309N1/mus309D2 larvae.

Genunspecified/Df(3L)Exel6103 mus309N1/mus309D2 spn-Aunspecified triple mutant larvae have small brain and generally lack imaginal discs (although very small rudimentary discs are occasionally visible). Salivary gland imaginal ring cells are reduced in number and have enlarged nuclei. Mitotic neuroblasts have numerous chromosome breaks.

The addition of spn-Aunspecified suppresses the the chromosome breakage and polyploidy phenotypes seen in mitotic neuroblasts of mus309N1/mus309D2 mus312D1/mus312Z1973 larvae, and also suppresses the defects in the salivary gland imaginal ring cells seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants. However, addition of spn-Aunspecified does not suppress the pupal lethality, small brains and lack of imaginal discs seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants.

The presence of Lig4169/Lig4169 does not significantly enhance the severity of tumor formation, but does enhance the decreased lifespan seen in BlmN1/BlmN1 mutant adults.

mus312D1/mus312Z1973, mus309D2/mus309N1 double mutants are inviable, dying after pupariation. Third-instar larvae have melanotic tumors and lack larval imaginal discs. Larval brains of double mutants are greatly reduced in size.

The spn-A3/spn-A093A transheterozygous condition does not suppress the lethality of mus312D1/mus312Z1973, mus309D2/mus309N1 double mutants.

Apoptosis in the wing disc is highly elevated compared to wild type in mus81Nhe ; mus309N1 double mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (12)