FlyBase Allele Report: Dmel\spn-A[unspecified]

General Information

Symbol

Dmel\spn-A^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0086328

Feature type

allele

Associated gene

Dmel\spn-A

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.

(FlyBase Curators, 2025-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage

(Klovstad et al., 2008, Tearle and Nusslein-Volhard, 1987)

egg

(Tearle and Nusslein-Volhard, 1987)

karyosome

(Ghabrial et al., 1998)

Detailed Description

Statement

Reference

spn-A^unspecified females lay eggs which show patterning defects, including having a single dorsal appendage, and having no dorsal appendages, being completely ventralised.

(Klovstad et al., 2008)

Excision and repair events of P{hsw^a} in developing mitotic germ line cells of spn-A^unspecified males carrying P{hsw^a} and a source of transposase have been detected by crossing the males to females homozygous for P{hsw^a}. No red eyed females have been detected in the progeny. Red-eyed progeny are produced by synthesis-dependent strand annealing (SDSA) with annealing at the LTRs of the copia element present in the P{hsw^a} transgene. The lack of red-eyed female progeny indicates that spn-A is required for SDSA. Assaying repair of double strand breaks caused by excision of P{hsw^a} in spn-A^unspecified heterozygous males indicates show a prominent defect in the ability to complete SDSA repair; the % of red-eyed progeny (representing repair by SDSA) from spn-A^unspecified heterozygous males is reduced by 50% relative to wild type), whereas the % of yellow-eye progeny (representing aberrant repair) is increased.

(McVey et al., 2004)

Condensation of DNA in the oocyte nucleus to form the karyosome is abnormal in homozygous egg chambers. In some cases the DNA is more diffuse than normal or it is threadlike and fragmented.

(Ghabrial et al., 1998)

The shape of eggs derived from spn-A^unspecified mutant females is abnormal. In extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity. Some eggs have one fused dorsal appendage. Eggs are often slightly collapsed.

(Tearle and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

spn-A^unspecified is a suppressor of lethal | first instar larval stage phenotype of Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103

(Andersen et al., 2011)

NOT Suppressor of

Statement

Reference

spn-A^unspecified is a non-suppressor of lethal | pupal stage phenotype of Blm^N1/Blm^D2, mus312^D1/mus312^Z1973

(Andersen et al., 2011)

Other

Statement

Reference

Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103, spn-A^unspecified has lethal | pupal stage phenotype

(Andersen et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

spn-A^unspecified has dorsal appendage phenotype, suppressible | partially by Brca2^KO/Brca2^56E

(Klovstad et al., 2008)

Suppressor of

Statement

Reference

spn-A^unspecified is a suppressor of condensed chromosome phenotype of Blm^N1/Blm^D2, mus81^Nhe

(Andersen et al., 2011)

spn-A^unspecified is a suppressor of condensed chromosome phenotype of Blm^N1/Blm^D2, mus312^D1/mus312^Z1973

(Andersen et al., 2011)

spn-A^unspecified is a suppressor of salivary gland imaginal ring phenotype of Blm^N1/Blm^D2, mus312^D1/mus312^Z1973

(Andersen et al., 2011)

NOT Suppressor of

Statement

Reference

spn-A^unspecified is a non-suppressor of larval brain phenotype of Blm^N1/Blm^D2, mus312^D1/mus312^Z1973

(Andersen et al., 2011)

spn-A^unspecified is a non-suppressor of imaginal disc | larval stage phenotype of Blm^N1/Blm^D2, mus312^D1/mus312^Z1973

(Andersen et al., 2011)

Other

Statement

Reference

Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103, spn-A^unspecified has larval brain phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103, spn-A^unspecified has salivary gland imaginal ring phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103, spn-A^unspecified has imaginal disc | larval stage phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, Gen^unspecified/Df(3L)Exel6103, spn-A^unspecified has condensed chromosome phenotype

(Andersen et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of spn-A^unspecified significantly decreases the number of chromosome breaks seen in mitotic neuroblasts of mus81^Nhe ; mus309^N1/mus309^D2 larvae.

Gen^unspecified/Df(3L)Exel6103 mus309^N1/mus309^D2 spn-A^unspecified triple mutant larvae have small brain and generally lack imaginal discs (although very small rudimentary discs are occasionally visible). Salivary gland imaginal ring cells are reduced in number and have enlarged nuclei. Mitotic neuroblasts have numerous chromosome breaks.

The addition of spn-A^unspecified suppresses the the chromosome breakage and polyploidy phenotypes seen in mitotic neuroblasts of mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 larvae, and also suppresses the defects in the salivary gland imaginal ring cells seen in the mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 double mutants. However, addition of spn-A^unspecified does not suppress the pupal lethality, small brains and lack of imaginal discs seen in the mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 double mutants.

(Andersen et al., 2011)

The eggshell patterning defects of eggs laid by spn-A^unspecified females are partially suppressed by Brca2^KO/Brca2^56E.

(Klovstad et al., 2008)

The phenotype of rec^unspecified spn-A^unspecified double mutants is similar to that of spn-A^unspecified single mutants.

(Blanton et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

spn-A^unspecified

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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