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General Information
Symbol
Dmel\spn-Aunspecified
Species
D. melanogaster
Name
FlyBase ID
FBal0086328
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    spn-Aunspecified females lay eggs which show patterning defects, including having a single dorsal appendage, and having no dorsal appendages, being completely ventralised.

    Excision and repair events of P{hswa} in developing mitotic germ line cells of spn-Aunspecified males carrying P{hswa} and a source of transposase have been detected by crossing the males to females homozygous for P{hswa}. No red eyed females have been detected in the progeny. Red-eyed progeny are produced by synthesis-dependent strand annealing (SDSA) with annealing at the LTRs of the copia element present in the P{hswa} transgene. The lack of red-eyed female progeny indicates that spn-A is required for SDSA. Assaying repair of double strand breaks caused by excision of P{hswa} in spn-Aunspecified heterozygous males indicates show a prominent defect in the ability to complete SDSA repair; the % of red-eyed progeny (representing repair by SDSA) from spn-Aunspecified heterozygous males is reduced by 50% relative to wild type), whereas the % of yellow-eye progeny (representing aberrant repair) is increased.

    Condensation of DNA in the oocyte nucleus to form the karyosome is abnormal in homozygous egg chambers. In some cases the DNA is more diffuse than normal or it is threadlike and fragmented.

    The shape of eggs derived from spn-Aunspecified mutant females is abnormal. In extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity. Some eggs have one fused dorsal appendage. Eggs are often slightly collapsed.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    NOT Suppressor of
    Statement
    Reference
    Other
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The addition of spn-Aunspecified significantly decreases the number of chromosome breaks seen in mitotic neuroblasts of mus81Nhe ; mus309N1/mus309D2 larvae.

    Genunspecified/Df(3L)Exel6103 mus309N1/mus309D2 spn-Aunspecified triple mutant larvae have small brain and generally lack imaginal discs (although very small rudimentary discs are occasionally visible). Salivary gland imaginal ring cells are reduced in number and have enlarged nuclei. Mitotic neuroblasts have numerous chromosome breaks.

    The addition of spn-Aunspecified suppresses the the chromosome breakage and polyploidy phenotypes seen in mitotic neuroblasts of mus309N1/mus309D2 mus312D1/mus312Z1973 larvae, and also suppresses the defects in the salivary gland imaginal ring cells seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants. However, addition of spn-Aunspecified does not suppress the pupal lethality, small brains and lack of imaginal discs seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants.

    The eggshell patterning defects of eggs laid by spn-Aunspecified females are partially suppressed by Brca2KO/Brca256E.

    The phenotype of recunspecified spn-Aunspecified double mutants is similar to that of spn-Aunspecified single mutants.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    spn-Aunspecified
    Name Synonyms
    Secondary FlyBase IDs
      References (7)