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General Information
Symbol
Dmel\mus312Z1973
Species
D. melanogaster
Name
FlyBase ID
FBal0150331
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A7330095T

Amino acid change:

K143term | mus312-PA; K143term | mus312-PD; K143term | mus312-PE

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: K143term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

X-ray irradiation of wild-type third-instar larvae induces cell cycle arrest within 1 hour. In contrast, X-ray irradiation of mus312Z1973/mus312D1 mutant larvae fails to induce cell cycle arrest.

Mutant larvae are sensitive to nitrogen mustard and to methyl methanesulfonate (compared to control larvae).

mus312Z1973 homozygotes much lower meiotic crossover frequency than wild-type flies. Survival of mus312D1/mus312Z1973 or mus312Z1973 homozygous adults treated with DNA damaging agent Nitrogen mustard, is significantly reduced compared to wild-type. mus312Z1973/mus312D1 animals have an X chromosome non-disjunction rate of 24%.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Spontaneous mitotic crossovers are completely absent in Fancm0693/Df(3R)ED6058 animals that are also mutant for mus312D1/mus312Z1973.

mus309N1/mus309D2 mus312D1/mus312Z1973 double mutant larvae have small brains, lack imaginal discs and have a reduced number of salivary gland imaginal ring cells. The nuclei of the remaining salivary gland imaginal ring cells appear larger than normal. Mitotic neuroblasts in these larvae show extreme genome instability; no mitotic nuclei with completely intact chromosomes are detected and approximately one-third show polyploidy.

The addition of spn-Aunspecified suppresses the the chromosome breakage and polyploidy phenotypes seen in mitotic neuroblasts of mus309N1/mus309D2 mus312D1/mus312Z1973 larvae, and also suppresses the defects in the salivary gland imaginal ring cells seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants. However, addition of spn-Aunspecified does not suppress the pupal lethality, small brains and lack of imaginal discs seen in the mus309N1/mus309D2 mus312D1/mus312Z1973 double mutants.

mus309D2/mus309N2 mus312D1/mus312Z1973 double mutant larvae generally lack imaginal discs (although small, severely underdeveloped discs are sometimes seen in third instar larvae).

mus312D1/mus312Z1973, mus309D2/mus309N1 double mutants are inviable, dying after pupariation. Third-instar larvae have melanotic tumors and lack larval imaginal discs. Larval brains of double mutants are greatly reduced in size.

The spn-A3/spn-A093A transheterozygous condition does not suppress the lethality of mus312D1/mus312Z1973, mus309D2/mus309N1 double mutants.

The increased lethality in response to nitrogen mustard treatment of mus312Z1973 homozygotes is not enhanced by homozygosity for mei-9A2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Laurencon, Hawley and Burtis

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)