FlyBase Allele Report: Dmel\Fancm[0693]

General Information

Symbol

Dmel\Fancm⁰⁶⁹³

Species

D. melanogaster

Name

FlyBase ID

FBal0300932

Feature type

allele

Associated gene

Dmel\Fancm

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: T?A.

(Sekelsky, 2015.5.1)

Amino acid replacement: L?term.

(Sekelsky, 2015.5.1)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,482,688..21,482,688 [+]

Nucleotide change:

T21482688A

Reported nucleotide change:

T?A

Amino acid change:

L82term | Fancm-PA; L82term | Fancm-PB

Reported amino acid change:

L?term

Comment:

The mutated base is the first base in the sequence TGGGAAAGACCTTCATTGCGGCGGTGGTTATGTACAACTTTT.

(Sekelsky, 2015.5.1)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair (with Df(3R)ED6058)

(Kuo et al., 2014)

abnormal meiotic cell cycle (with Df(3R)ED6058)

(Kuo et al., 2014)

abnormal mitotic cell cycle (with Df(3R)ED6058)

(Kuo et al., 2014)

abnormal mitotic cell cycle (with Fancm^del)

(Romero et al., 2016)

chemical sensitive (with Df(3R)ED6058)

(Kuo et al., 2014)

radiation sensitive (with Df(3R)ED6058)

(Kuo et al., 2014)

radiation sensitive (with Fancm^del)

(Romero et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Fancm⁰⁶⁹³/Fancm^del transheterozygous flies display significant increase in the number of spontaneous mitotic crossovers (assayed in males) and are sensitive gamma irradiation (low survival rate).

(Romero et al., 2016)

Fancm⁰⁶⁹³/Df(3R)ED6058 animals are hyper-sensitive to the cross-linking agent HN2, the mono-adduct generator MMS, and ionizing radiation.

Meiotic crossovers are elevated in Fancm⁰⁶⁹³/Df(3R)ED6058 animals, though only in a subset of the genome (centromere-proximal intervals). This elevation does not appear to have any negative impact on chromosome segregation.

Spontaneous mitotic crossovers are elevated in Fancm⁰⁶⁹³/Df(3R)ED6058 males.

Fancm⁰⁶⁹³/Df(3R)ED6058 animals show a decreased ability to complete 'synthesis-dependent strand annealing' repair of a DNA gap.

(Kuo et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal DNA repair phenotype, enhanceable by Blm^N1/Blm^D2

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has chemical sensitive phenotype, enhanceable by Blm^N1/Blm^D2

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has radiation sensitive phenotype, enhanceable by Blm^N1/Blm^D2

(Kuo et al., 2014)

Suppressed by

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, suppressible by mus312^D1/mus312^Z1973

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, suppressible by slx1^F93I/mus81^Nhe

(Kuo et al., 2014)

NOT suppressed by

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, non-suppressible by Gen^Z5997

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, non-suppressible by mus81^Nhe

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, non-suppressible by slx1^F93I

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, non-suppressible by mei-9^a

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 has abnormal mitotic cell cycle phenotype, non-suppressible by slx1^F93I/mei-9^a

(Kuo et al., 2014)

Enhancer of

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058 is an enhancer of abnormal DNA repair phenotype of Blm^N1/Blm^D2

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 is an enhancer of chemical sensitive phenotype of Blm^N1/Blm^D2

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058 is an enhancer of radiation sensitive phenotype of Blm^N1/Blm^D2

(Kuo et al., 2014)

Other

Statement

Reference

Blm^N1/Blm^D2, Fancm⁰⁶⁹³/Df(3R)ED6058 has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997 has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973 has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus81^Nhe has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, slx1^F93I has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mei-9^a has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mei-9^a, slx1^F93I has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus312^D1/mus312^Z1973 has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus81^Nhe, slx1^F93I has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus312^D1/mus312^Z1973, mus81^Nhe has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority die phenotype

(Kuo et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The rate of spontaneous mitotic crossovers in Blm^N1/Blm^D2 Fancm⁰⁶⁹³/Df(3R)ED6058 males is not significantly different from that in Fancm⁰⁶⁹³/Df(3R)ED6058 males, but is significantly lower than in Blm^N1/Blm^D2 males.

The rate of spontaneous mitotic crossovers in Fancm⁰⁶⁹³/Df(3R)ED6058 males is not affected if animals are also mutant for Gen^Z5997, mus81^Nhe, slx1^F93I or mei-9^a, or if these animals are also mutant for both slx1^F93I and mei-9^a. However crossovers are completely or nearly absent in Fancm⁰⁶⁹³/Df(3R)ED6058 animals that are also mutant for mus312^D1/mus312^Z1973, or are also mutant for both mus81^Nhe and slx1^F93I.

(Kuo et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fancm⁰⁶⁹³/Fancm^del is rescued by Fancm^+tFL

(Romero et al., 2016)

Partially rescued by

Fancm⁰⁶⁹³/Fancm^del is partially rescued by Fancm^FLKM

(Romero et al., 2016)

Fancm⁰⁶⁹³/Fancm^del is partially rescued by Fancm^1-645

(Romero et al., 2016)

Not rescued by

Fancm⁰⁶⁹³/Fancm^del is not rescued by Fancm^1-645.K84M

(Romero et al., 2016)

Comments

The increased number of mitotic crossovers as well as the low survival rate upon irradiation characteristic for Fancm⁰⁶⁹³/Fancm^del flies is rescued to a varying degree by combination with Fancm^+tFL or Fancm^FLKM or Fancm^1-645 but cannot be rescued by combination with Fancm^1-645.K84M.

(Romero et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Fancm⁰⁶⁹³

(Romero et al., 2016, Kuo et al., 2014)

fancm⁰⁶⁹³

(Thomas et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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