FlyBase Allele Report: Dmel\Tl[10b.UAS]

General Information

Symbol

Dmel\Tl^10b.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0098792

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

P{UAS-Tl.10b}

Also Known As

UAS-Toll^10b, UAS-Tl^10b, UAS-Toll10B

Key Links

Transgenic product class

missense_variant

(Maxton-Kuchenmeister et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Maxton-Kuchenmeister et al., 1999)

Mutagen

in vitro construct

(Maxton-Kuchenmeister et al., 1999)

Progenitor genotype

Tl^10b

(Maxton-Kuchenmeister et al., 1999)

Carried in construct

P{UAS-Tl.10b}

Cytology

Description

UAS regulatory sequences drive expression of full length Tl^10b sequences.

(Maxton-Kuchenmeister et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of collagen disease

CEA

(Zang et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of collagen disease

is ameliorated by PH4αEFB^GD771

(Zang et al., 2015)

ameliorates hematologic cancer

modeled by mxc¹

(Araki et al., 2019)

exacerbates carcinoma

modeled by scrib¹

(Katsukawa et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

decreased cell death | embryonic stage 17, with Scer\GAL4^elav-C155

(Zhu et al., 2008)

decreased cell number | third instar larval stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

increased cell death | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

increased cell number | larval stage, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

Phenotype Manifest In

embryonic/larval crystal cell, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

filzkorper, with Scer\GAL4^{VP16.mat.αTub67C}

(Maxton-Kuchenmeister et al., 1999)

hemocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

lamellocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

larval sessile hemocyte, with Scer\GAL4^Hml.Δ

(Valanne et al., 2010)

larval ventral nerve cord, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

larval ventral nerve cord | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

melanotic mass, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

melanotic mass | larval stage, with Scer\GAL4^r4

(Zang et al., 2015)

neuron | third instar larval stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

plasma membrane, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Maxton-Kuchenmeister et al., 1999)

Detailed Description

Statement

Reference

Expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^elav.PU results in significantly decreased number of eve-positive neurons in the ventral nerve cord (VNC) in third instar larvae along with increased number of apoptotic (Dcp-1-positive) cells in the pupal VNC, as compared to driver-only controls.

(Foldi et al., 2017)

Third larval instar adipocytes expressing Tl^10b.Scer\UAS under the control of Scer\GAL4^{SPARC-MI00329-GAL4} show a significant increase in both plasma membrane depth and sinuosity compared to control cells.

Expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in elongation of the ventral nerve cord.

(Zang et al., 2015)

Expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^Cg.PA results in the growth of chronic inflammatory hematopoietic tumours in larvae.

(Gueguen et al., 2013)

Larvae expressing Tl^10b.Scer\UAS under the control of Scer\GAL4^Hml.Δ show a loss of the sessile hemocyte banding pattern which is seen in wild-type larvae.

(Valanne et al., 2010)

Expression of Tl^10b.Scer\UAS in all neurons (under the control of Scer\GAL4^elav-C155) rescues naturally occuring cell death at embryonic stage 17 (but not at stages 13 and 14), illustrating that Tl can maintain neuronal survival.

(Zhu et al., 2008)

When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation and lamellocyte numbers are increased and crystal cells are unaffected. Melanotic masses form.

(Zettervall et al., 2004)

Expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} during the syncytial blastoderm stage results in ventralised embryos; the denticle belts form circumferential rings and the Filzkorpers are strongly reduced or absent.

(Maxton-Kuchenmeister et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)ro80b, Tl^10b.UAS has increased cell death phenotype, enhanceable by spz²

(Zhu et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^r4, Tl^10b.UAS has melanotic mass phenotype | larval stage phenotype, suppressible by Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4

(Zang et al., 2015)

Scer\GAL4^r4, Tl^10b.UAS has melanotic mass phenotype | larval stage phenotype, suppressible by PH4αEFB^GD771, Scer\GAL4^r4

(Zang et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{SPARC-MI00329-GAL4}, Tl^10b.UAS has plasma membrane phenotype, suppressible by Dif^HM05191, Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

Scer\GAL4^{SPARC-MI00329-GAL4}, Tl^10b.UAS has plasma membrane phenotype, suppressible by Rab1^JF02609, Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

Scer\GAL4^r4, Tl^10b.UAS has melanotic mass | larval stage phenotype, suppressible by Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4

(Zang et al., 2015)

Scer\GAL4^r4, Tl^10b.UAS has melanotic mass | larval stage phenotype, suppressible by PH4αEFB^GD771, Scer\GAL4^r4

(Zang et al., 2015)

Scer\GAL4^Hml.Δ, Tl^10b.UAS has larval sessile hemocyte phenotype, suppressible by Gprk2^NIG.17998R, Scer\GAL4^Hml.Δ

(Valanne et al., 2010)

Scer\GAL4^Hml.Δ, Tl^10b.UAS has larval sessile hemocyte phenotype, suppressible by Myd88^GD9716, Scer\GAL4^Hml.Δ

(Valanne et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in plasma membrane depth seen in third larval instar adipocytes expressing Tl^10b.Scer\UAS under the control of Scer\GAL4^{SPARC-MI00329-GAL4} is suppressed by co-expression of either Rab1^JF02609 or Dif^HM05191.

Simultaneous co-expression of both vkg^NIG.16858R and Cg25C^GD12784 suppresses the melanotic phenotype caused by expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^r4.

Co-expression of PH4αEFB^GD771 suppresses the melanotic phenotype caused by expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^r4.

(Zang et al., 2015)

Co-expression of either Gprk2^NIG.17998R or Myd88^GD9716 suppresses the loss of the sessile hemocyte banding pattern which is seen in larvae expressing Tl^10b.Scer\UAS under the control of Scer\GAL4^Hml.Δ.

(Valanne et al., 2010)

Levels of apoptosis increase in the CNS of spz² and Tl^r3/Df(3R)ro80b mutant embryos, indicating that both spz and Tl are required for neuronal survival.

(Zhu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of either Zzzz\Pvank1^{Scer\UAS.T:Zzzz\FLAG} or Zzzz\I2vank3^{Scer\UAS.T:Zzzz\FLAG} results in a decrease in both size and number of hematopoietic tumours caused by expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^Cg.PA.

(Gueguen et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58987

P{UAS-Tl.10b}11, y¹ w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tl^10b.Scer\UAS

Tl^10b.UAS

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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