FlyBase Allele Report: Dmel\Col4a1[GD12784]

General Information

Symbol

Dmel\Col4a1^GD12784

Species

D. melanogaster

Name

FlyBase ID

FBal0206556

Feature type

allele

Associated gene

Dmel\Col4a1

Associated Insertion(s)

Carried in Construct

P{GD12784}

Also Known As

UAS-Cg25C.RNAi^VDRC.v28369, UAS-Cg25C-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12784}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12784

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

At a permissive temperature (18[o]C) expression of Avic\GFP^{dsRNA.R.Scer\UAS} under the control of Scer\GAL4^Cg.PA and Scer\GAL80^ts.αTub84B knocks down expression of vkg^G454. These flies develop into normal adults, although pupal lethality results when the larvae are transferred to restrictive temperature after completion of embryogenesis. Dissection of these larvae 96 hours after the temperature shift reveals highly aberrant shapes in several organs. Imaginal discs, consisting of highly columnar epithelial cells, appear flattened while the ventral nerve cord portion of the central nervous system is strikingly elongated, reminiscent of embryonic defects in ventral nerve cord condensation. Additionally, the ducts and imaginal rings of the salivary glands are dilated, resulting in an expanded lumen.

(Pastor-Pareja and Xu, 2011)

Adults expressing Cg25C^GD12784 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12784

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass phenotype | larval stage phenotype of Scer\GAL4^r4, shi^NIG.18102R

(Zang et al., 2015)

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass phenotype | larval stage phenotype of Scer\GAL4^r4, Tl^10b.UAS

(Zang et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of embryonic/larval fat body | larval stage phenotype of Scer\GAL4^r4, shi^NIG.18102R

(Zang et al., 2015)

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass | larval stage phenotype of Scer\GAL4^r4, Tl^10b.UAS

(Zang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous co-expression of both vkg^NIG.16858R and Cg25C^GD12784 suppresses the melanotic fat body phenotype caused by expression of shi^NIG.18102R under the control of Scer\GAL4^r4.

Simultaneous co-expression of both vkg^NIG.16858R and Cg25C^GD12784 suppresses the melanotic phenotype caused by expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^r4.

(Zang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v28369

w¹¹¹⁸; P{GD12784}v28369

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12784

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cg25C^GD12784

Cg25C^RNAi

(Csordás et al., 2020)

Col4a1^GD12784

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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