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General Information
Symbol
Dmel\spz2
Species
D. melanogaster
Name
FlyBase ID
FBal0016060
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
spz197
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T27066334A

Reported nucleotide change:

T400A

Amino acid change:

Y190N | spz-PA; Y164N | spz-PB; Y117N | spz-PC; Y137N | spz-PD; Y91N | spz-PI; Y108N | spz-PJ; Y154N | spz-PK; Y134N | spz-PL

Reported amino acid change:

Y134N

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Y134N.

Nucleotide substitution: T400A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

VA1d olfactory receptor neuron axon targeting is normal in mutant flies.

spz2 mutant embryos exhibit aberrant targeting by the SNa motor axons.

spz2 flies can barely walk.

Hemolymph clots from spz2 third instar larvae show normal melanization.

Homozygous females produce dorsalised embryos.

Level of Drs induction of bacterially challenged mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of spz4/spz2 mutants with A.fumigatus causes 3% survival 3 days postinfection, infection with E.coli causes 84% survival 3 days postinfection.

Injection of snkΔne.T:ea into the central region of homozygous embryos causes a dorsalized phenotype.

Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements.

Embryos derived from spz2/spz3 females are weakly dorsalised at 18oC. The mesoderm and ventral furrow are reduced in size, and mesoderm only develops in the posterior part of the embryo.

Embryos derived from homozygous females are dorsalised.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

spz2 has increased cell death phenotype, non-enhanceable by NT141

Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

spz2 is a non-enhancer of abnormal neuroanatomy phenotype of NT141

spz2 is a non-enhancer of increased cell death phenotype of NT141

spz2 is a non-enhancer of visible phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

spz2 is a non-suppressor of visible phenotype of upd1GMR.PB

Other
Phenotype Manifest In
Enhanced by
Enhancer of
NOT Enhancer of
Statement
Reference

spz2 is a non-enhancer of segmental nerve phenotype of NT141

spz2 is a non-enhancer of eye phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

spz2 is a non-suppressor of eye phenotype of upd1GMR.PB

Additional Comments
Genetic Interactions
Statement
Reference

Pan-neuronal expression of Tl10b.Scer\UAS.cYa, under the control of Scer\GAL4elav-C155, suppresses the semi-lethality seen in spz2 mutants.

Pan-neuronal expression of Toll-6C1020Y.Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4elav-C155, suppresses the semi-lethality seen in spz2 mutants.

Pan-neuronal expression of Toll-7C993Y.Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4elav-C155, suppresses the semi-lethality seen in spz2 mutants.

The SNa axonal phenotype of spz2 NT141 double-mutant embryos is epistatic to spz.

Levels of apoptosis increase in the CNS of spz2 and Tlr3/Df(3R)ro80b mutant embryos, indicating that both spz and Tl are required for neuronal survival.

Levels of apoptosis do not increase further in spz2 NT141 double mutants (compared to spz2 mutants).

Whereas homozygous spz2 flies can eclose as adults, the NT141 spz2 double mutants are completely lethal (100% penetrance).

The penetrance of both SNa and ISNb/d targeting defects increases in spz5e03444 NT141 spz2, compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%).

NT141 spz5e03444 / NT141 Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.

Perivitelline fluid from embryos laid by Tlrv13 spz4/Tlrv4 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD3 spz4/ea1 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD1 spz2/ea4 spz4 females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip1/pip2 females.

The zygotic semi-lethality of cact7 homozygotes is not suppressed by spz2.

Double mutant combinations with eaD1 are strongly dorsalizing.

Xenogenetic Interactions
Statement
Reference

The presence of one copy of spz2 markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2BIntron5.Scer\UAS under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Partially rescued by

spz2 is partially rescued by spzp4-8.23

spz2 is partially rescued by spzp4-8.29

spz2 is partially rescued by spzp4-8.20

spz2 is partially rescued by spzp4-11.32

spz2 is partially rescued by spzp4-8.24

spz2 is partially rescued by spzp4-11.27

spz2 is partially rescued by spzp4-11.6

spz2 is partially rescued by spzp4-11.5

Comments

Microinjection of purified spz2.1 protein into the perivitelline space of syncytial blastoderm stage embryos rescues embryonic phenotype: restores the ventrolateral pattern elements and normalises the dorsal-ventral axis of the recipient embryo.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (31)