FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pdk1UAS.Tag:FLAG
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General Information
Symbol
Dmel\Pdk1UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0265720
Feature type
allele
Associated gene
Dmel\Pdk1
Associated Insertion(s)
Carried in Construct
P{UAS-Pdk1.FLAG}
Key Links
Transgenic product class
wild_type
(Cheng et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Cheng et al., 2011)
Mutagen
in vitro construct
(Cheng et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Pdk1.FLAG}
Cytology
Description

UAS regulatory sequences drive expression of the Pdk1 open reading frame which is tagged at the N-terminal end with three copies of Tag:FLAG.

(Cheng et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pdk1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4VGlut-OK371 or Scer\GAL4tub.PU increases the absolute bouton number at the third larval instar neuromuscular junction.

      Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4VGlut-OK371 results in an increase in the number of active zones, an increase in the total synaptic area and a small increase in active zone density at the third larval instar neuromuscular junction.

      (Cheng et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4VGlut-OK371 does not rescue the reduction in bouton size which is seen at the neuromuscular junction in S6kl-1/Df(3L)CH18 third instar larvae.

      (Cheng et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4VGlut1-OK371/Pdk1UAS.Tag:FLAG partially rescues Pdk133

      (Cheng et al., 2011)

      Pdk1UAS.Tag:FLAG/Scer\GAL4C57 partially rescues Pdk133

      (Cheng et al., 2011)
      Comments

      Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4VGlut-OK371 fully restores the defects in bouton size which are seen at the Pdk133 neuromuscular junction, but the defects in muscle size that are also seen at the Pdk133 neuromuscular junction are not rescued.

      Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C57 fully restores the defects in muscle size which are seen at the Pdk133 neuromuscular junction, but the defects in bouton size that are also seen at the Pdk133 neuromuscular junction are not rescued.

      (Cheng et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pdk1Scer\UAS.T:Zzzz\FLAG
      Pdk1UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)