FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lkb1, Pdk1
FlyBase ID
FBig0000121968
Interaction Network
Interactions Browser links
Lkb1 network
Pdk1 network
Reported Interactions
FBrf0256005-1.coIP.WB
Reference
Borkowsky et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Lkb1 

LKB1

bait

GFP tag

Lkb1 
Pdk1 

PDK1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lkb1 
interaction residue 253
mutation decreasing interaction
aa 253

E253A; coordinates relative to Lkb1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Borkowsky et al., 2023)
FBrf0256005-2.coIP.WB
Reference
Borkowsky et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Lkb1 

LKB1

bait

GFP tag

Lkb1 
Pdk1 

PDK1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lkb1 
interaction residue 353
mutation increasing interaction
aa 353

T253A; coordinates relative to Lkb1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Borkowsky et al., 2023)
FBrf0256005-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pdk1 

PDK1

enzyme
Pdk1 

PDK1

enzyme
Lkb1 

LKB1

enzyme target

MBP tag

Lkb1 

LKB1

enzyme target

MBP tag

Lkb1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 0 )
References (1)