TAAGACCCGAGTCTTCGTGTATACGTATAATTACCATTTGCAATCACAAAGGTTTTGCAACTGTCGCCGGGCGGTGAAAA CTCAAGTGAATAAATATCGCAAAGTGAAGCAAATAAAAAAATATTACAAAAACCGGTAAAACGAAATGAAACTGAAATGA GTGAGGGCAGCAACTTCAAGTGACGAAAGCAATCCGTTTACCGTTGTCTTTGCCTCTTCATCCTCCCCGAGAACGGCGGA GAACGCGAAAAATATCATGAAATGTAAAAGCTGGTCCAACAAAATTAACAACTACGTTGTGCGCAAAATAAAGTCTATCA AGATCAACGGTACACAGCAACAGCTCCAATTGCCAGGCAGCGGCGCTAGCGGAATCGCAGCGGCAGCCGTAATCACGGTA GCATCGGATTGCGGCGAAAACTGCAGCAGTAACGGCACCGAGCATCAGCAGCACTTCAACATTGCTACCACCACAGCAAC TTCGGCGACAGAGGCAACAATGCCGGCTATGGCCAAGGAGAAAGCATCAGCAACAGTGTCCTTGGGCGAGTCGAATTTCA GAGATATCAACCTAAAAGACTTGGCCGTGGTCGTCGAAGCGGCATCGCGGCTGCACCACCAGCAAAACGTATGCGGCTGC GGAGCGGTATCGTCTACTGAGAACAACAACAACAGTCGCTACGGCAGCAGCAAGTATCTGACAAACGGCCACACGTCGCC TTTGGCGGCAGCGGTTGCTAGCAACAGTTCGTCGGTGGCCACGACACCGCATTGCAGAATGTTGCACAACTGCAGTCTGC AGCAGTACCAGAATGACATAAGGCAGCAGACGGAGATATTGGACATGTTGCGGCAGCAGCATCAGCAGGGCTACCAGTCG CAGCAACAGCAACAACAGCCGCAGCAGCAGCAGGAACAACAGCAGCAGCAGGAGCAATCGCAGCATCAGCAACAGCTACA GAATCCTGCGCCCAGGAGGTCTCCGAATGATTTCATTTTCGGTCGTTACATAGGCGAGGGCAGCTATAGCATAGTTTATC TGGCCGTGGATATACACTCTCGCCGCGAGTATGCAATTAAAGTATGCGAGAAGCGGCTGATCCTGCGCGAACGGAAGCAG GACTACATCAAGCGTGAACGCGAGGTGATGCACCAGATGACCAACGTTCCCGGCTTCGTAAACCTGTCGTGCACCTTCCA GGACCAGCGTTCTCTTTACTTTGTGATGACGTACGCGCGAAAGGGCGACATGTTGCCATACATCAACCGCGTGGGTAGCT TTGACGTGGCCTGCACGCGCCACTACGCTGCCGAGCTTCTGCTGGCCTGCGAGCACATGCACCGCCGTAATGTGGTGCAC CGCGACCTCAAGCCCGAGAACATCCTGCTCGACGAGGACATGCACACGCTAATCGCCGACTTCGGTTCCGCCAAGGTGAT GACAGCCCACGAAAGGGCTCTGGCCACGGAGCACTGTTCGGAGCAGCGGCGCAGCAACTCCGATGAAGACGATGAAGACA GCGACCGCCTGGAAAACGAAGACGAAGACTTCTACGATCGCGATTCGGAGGAGTTGGACGACGGCGACGACGAACAGCAG CAGGAAGAGATGGACTCCCCACGCCATCGTCAAAGGCGTTACAACCGTCACCGAAAGGCAAGCTTTGTGGGCACTGCCCA GTACGTTTCACCGGAAGTGCTCCAAAATGGACCTATAACCCCGGCGGCGGACCTGTGGGCACTGGGATGTATAGTTTATC AGATGATCGCCGGCCTACCGCCATTCCGTGGCAGCAACGATTATGTCATTTTTAAAGAGATTCTTGACTGCGCCGTGGAC TTCCCGCAAGGCTTTGATAAGGACGCCGAGGACCTGGTGCGCAAGCTGCTCCGTGTCGATCCGCGAGACCGCCTGGGTGC TCAGGACGAGTTTGGATATTACGAATCCATTCGGGCGCACCCATTCTTCGCCGGCATTGACTGGCAGACGCTCCGTCAAC AAACCCCTCCCCCCATCTACCCATACTTACCGGGCGTAAGTCAGGATGAGGACTTCAGGTCCAGCTATACTGTGCCCGGG GATTTGGAGCCTGGCCTTGACGAACGGCAGATATCGCGACTGCTTAGTGCAGAATTAGGCGTTGGCAGCAGCGTGGCCAT GCCCGTCAAGCGATCGACCGCAAAGAACTCTTTCGACCTGAACGATGCCGAGAAGCTGCAGCGTCTCGAGCAACAAAAGA CTGACAAGTGGCACGTTTTTGCCGACGGCGAAGTGATCCTCAAGAAGGGGTTTGTCAACAAGCGCAAGGGTCTGTTTGCC CGCAAGCGAATGCTCCTTCTGACCACGGGACCGCGACTCATCTATATCGATCCCGTGCAGATGATCAAAAAAGGAGAAAT TCCGTGGAGCCCAGATCTCCGAGCGGAGTACAAAAACTTCAAAATTTTTTTTGTCCATACGCCAAATCGAACCTATTACC TGGATGATCCCGAAGGCTATGCTATCCACTGGTCGGAAGCTATTGAGAACATGCGCAAGTTGGCCTACGGAGATCCCTCC TCCACATCTGCAGTGTCCTGCTCCAGCGGCAGCAGTAATAGCCTGGCTGTCATCTCAAATTCATCCGCCGCCTCCTCAAG CAATTCGCCCACGGTGAAACGCAGTTCCCCCGTAAACGCTCCTCAAGCTTCGACGGCGTCTGACAACCGGACATTGGGTA GCACCAGAACGGGGACGTCACCTAGCAAGAAGACGGCGTCTAAGTAAACGTAGTCCTATTTATAGCAATGTGAAATTAAT TTAGTTGAAATTTAGTGCAAACGAAGCGATGGCGTAGAAGAGGGCGGGAATAGAAGTAAGCTTAGAAGTAGAAGTAAGTG ATGAAGGGGAAATAATGATCGTGTCTCTAGTGCTAATTAGAACCAAATTTCTGTTTTCCGATTTGTATTGTGCTTAGGGC GAAAATATAACACTAGAATAAACTTATCTAAAGTAAAATACATCTATAACGACTAAGTAAATTCAATTTAAAATTTAAAT TAAATATTTATATACAGCATACATAAATACAAATATATTCGTATTTAGCGCGTAGTTAGTCGCAATTGAAATGAATTACA CACGGAGGCAAGGTGGCATTGTAGGCTTAAATTAAAAGTAATTTCGTGAAGCAAACTAAAATCCGTATTTAAATGGTATA ATACTAGGAAATTATCTGGAAACTGTAATATACATAATATGTGTATGTACGTCGGAGAAATAATTTTTGTTTCTGTTATC GTGGGAAGATCACATATATGTTAATATAAATTTTGTAAACAGTGTAACTATGTACAGAACTATGTGAGCTATATGCACAC GGCAAAAAGACCCTAAGTCGTGGGGAAGGAGATGGGAATGAGCCGGAAAACCATCGATGTCACAAAATCGGTGTTTTTCC AGCTTCACGCTCAACTGTTTAGGTAATTCTAATATACAGTTTAACGCTAATTATCATTATTTATCATTCTCATTATGCTT TTCGTTTACCTATTTGCCCTATAATGAAATGTATAAGACTTGAGCTACCCTAATAAAAGAACGAATTTTAAAAAAAAAAA AAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
