FlyBase Allele Report: Dmel\Pdk1[A467V.UAS]

General Information

Symbol

Dmel\Pdk1^A467V.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0135871

Feature type

allele

Associated gene

Dmel\Pdk1

Associated Insertion(s)

Carried in Construct

P{UAS-Pdk1.A467V}

Also Known As

UAS-PDK1^A467V

Key Links

Transgenic product class

missense_variant

(Rintelen et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rintelen et al., 2001)

Mutagen

in vitro construct

(Rintelen et al., 2001)

Progenitor genotype

Pdk1^UAS.cRa

(Rintelen et al., 2001)

Carried in construct

P{UAS-Pdk1.A467V}

Cytology

Description

Construct: Nucleotide substitution: C2032T.

(Rintelen et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pdk1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size | third instar larval stage, with Scer\GAL4^AB1

(Sharma et al., 2019)

increased occurrence of cell division | larval stage, with Scer\GAL4^nab

(Sousa-Nunes et al., 2011)

visible, with Scer\GAL4^ap-md544

(Rintelen et al., 2001)

Phenotype Manifest In

fat cell | third instar larval stage, with Scer\GAL4^AB1

(Sharma et al., 2019)

larval neuroblast, with Scer\GAL4^nab

(Sousa-Nunes et al., 2011)

wing, with Scer\GAL4^ap-md544

(Rintelen et al., 2001)

Detailed Description

Statement

Reference

Third instar larvae expressing Pdk1^A467V.UAS under the control of Scer\GAL4^AB1 show significantly bigger salivary gland cells, as compared to controls.

(Sharma et al., 2019)

Central nervous system neuroblasts of fed larvae expressing Pdk1^{A467V.Scer\UAS} under the control of Scer\GAL4^nab show an increase in EdU incorporation compared to controls.

(Sousa-Nunes et al., 2011)

Expression of Pk61C^{A467V.Scer\UAS} under the control of Scer\GAL4^ap-md544 causes a bent-wing phenotype.

(Rintelen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^AB1 has increased cell size | third instar larval stage phenotype, non-enhanceable by PIP4K²⁹

(Sharma et al., 2019)

Suppressed by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Akt1[+]/Akt¹

(Rintelen et al., 2001)

Pdk1^A467V.UAS, Scer\GAL4^ap-md544 has visible phenotype, suppressible by S6k[+]/S6k^l-1

(Rintelen et al., 2001)

NOT suppressed by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^AB1 has increased cell size | third instar larval stage phenotype, non-suppressible by PIP4K²⁹

(Sharma et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^AB1 has fat cell | third instar larval stage phenotype, non-enhanceable by PIP4K²⁹

(Sharma et al., 2019)

Suppressed by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^ap-md544 has wing phenotype, suppressible by Akt1[+]/Akt¹

(Rintelen et al., 2001)

Pdk1^A467V.UAS, Scer\GAL4^ap-md544 has wing phenotype, suppressible by S6k[+]/S6k^l-1

(Rintelen et al., 2001)

NOT suppressed by

Statement

Reference

Pdk1^A467V.UAS, Scer\GAL4^AB1 has fat cell | third instar larval stage phenotype, non-suppressible by PIP4K²⁹

(Sharma et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pdk1^{A467V.Scer\UAS}

Pdk1^A467V.UAS

Pdk1^ACT

(Sousa-Nunes et al., 2011)

Pk61C^{A467V.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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