FlyBase Allele Report: Dmel\Pdk1[4]

General Information

Symbol

Dmel\Pdk1⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0135874

Feature type

allele

Associated gene

Dmel\Pdk1

Associated Insertion(s)

P{EP}EP837

Carried in Construct

Also Known As

EP837

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Rintelen et al., 2001)

Mutagen

ethyl methanesulfonate

(Rintelen et al., 2001)

P-element activity

(Rintelen et al., 2001)

Progenitor genotype

Pdk1^EP837

(Rintelen et al., 2001)

Associated Insertion(s)

P{EP}EP837

Cytology

Description

Amino acid replacement: P441L.

(Rintelen et al., 2001)

The progenitor P{EP}Pdk1^EP837 insertion is also still present.

(Rintelen et al., 2001)

Nucleotide substitution: C?T.

(Rintelen et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:142,385..142,385 [+]

Nucleotide change:

C142385T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

P441L

(Rintelen et al., 2001)

transposable_element_insertion_site

3L:131,762..131,762 [+]

(Rintelen et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Pdk1⁵)

(Rintelen et al., 2001)

decreased body size (with Pdk1⁵)

(Rintelen et al., 2001)

decreased body size | recessive | somatic clone

(Rintelen et al., 2001)

decreased cell size (with Pdk1⁵)

(Rintelen et al., 2001)

female sterile (with Pdk1⁵)

(Pallares-Cartes et al., 2012)

male sterile (with Pdk1⁵)

(Pallares-Cartes et al., 2012, Roote, 2003.9.11, Rintelen et al., 2001)

viable (with Pdk1⁵)

(Roote, 2003.9.11)

Phenotype Manifest In

adult head (with Pdk1⁵)

(Roote, 2003.9.11)

Detailed Description

Statement

Reference

Pk61C⁴/Pk61C⁵ surviving flies have reduced heads.

(Roote, 2003.9.11)

Pk61C⁵/Pk61C⁴ flies are delayed in development for one day, smaller than normal and have an 18% reduction in body weight. This reduction in size and weight is primarily due to a decrease in cell size. Pk61C⁵/Pk61C⁴ male flies are almost completely sterile, although they show no obvious defect in sperm morphology or in mating behaviour. The heads of mosaic flies with homozygous heads (generated using the "ey-FLP" system) are reduced in size.

(Rintelen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pdk1⁵/Pdk1⁴ has male sterile phenotype, suppressible by PRAS40^KO

(Pallares-Cartes et al., 2012)

Pdk1⁵/Pdk1⁴ has female sterile phenotype, suppressible by PRAS40^KO

(Pallares-Cartes et al., 2012)

Other

Statement

Reference

Pdk1⁵/Pdk1⁴, Pten^dj189/Pten^c494 has viable phenotype

(Rintelen et al., 2001)

Pdk1⁵/Pdk1⁴, Pten^dj189/Pten^c494 has visible phenotype

(Rintelen et al., 2001)

Phenotype Manifest In

Other

Statement

Reference

Pdk1⁵/Pdk1⁴, Pten^dj189/Pten^c494 has leg phenotype

(Rintelen et al., 2001)

Pdk1⁵/Pdk1⁴, Pten^dj189/Pten^c494 has adult abdomen phenotype

(Rintelen et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Pten^dj189/Pten^c494 ; Pk61C⁴/Pk61C⁵ animals can survive to adulthood, although the abdomen is unproportionally reduced in size and leg structures are deformed.

(Rintelen et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a suppressor of the big eye phenotype caused by coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF.

(Rintelen et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

PDK1⁴

(Pallares-Cartes et al., 2012)

Pdk1⁴

Pk61C⁴

dPDK1⁴

(Rintelen et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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