FlyBase Allele Report: Hsap\SNCA[UAS.cUa]

General Information

Symbol

Hsap\SNCA^UAS.cUa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0316592

Feature type

allele

Associated gene

Hsap\SNCA

Associated Insertion(s)

Carried in Construct

P{UAS-SNCA.U}

Also Known As

UAS-αSyn, UAS-α-syn

Key Links

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-SNCA.U}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\SNCA.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Liu et al., 2015, Yin et al., 2014)

model of Parkinson's disease 1

CEA

(Zhang et al., 2018, Merzetti et al., 2017, Büttner et al., 2013)

CEC

(Cunningham et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by Rab8^UASp.YFP

(Yin et al., 2014)

is exacerbated by aux^KK100927

(Song et al., 2017)

is exacerbated by aux^GD7187

(Song et al., 2017)

is exacerbated by Lk6^UAS.cUa

(Zhang et al., 2015)

model of Parkinson's disease 1

is ameliorated by EndoG^GD5747

(Büttner et al., 2013)

is ameliorated by Hsap\TMEM59^UAS.cLa

(Zhang et al., 2018)

is ameliorated by st^UAS.Venus

(Cunningham et al., 2018)

is ameliorated by ntc^JF01331

(Merzetti et al., 2017)

is exacerbated by PI31^GD13764

(Merzetti et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "Parkinson's disease 1" is associated with mutations in human SNCA.

(Cunningham et al., 2018)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Ddc.PU

(Liu et al., 2015)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Liu et al., 2015, Yin et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^elav.PU

(Zhang et al., 2015)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^ple.PF

(Cunningham et al., 2018)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^C164

(Yin et al., 2014)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^ple.PF

(Yin et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^Ddc.PU

(Liu et al., 2015)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Babcock et al., 2015)

abnormal neuroanatomy | progressive, with Scer\GAL4^elav.PU

(Zhang et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PF

(Cunningham et al., 2018)

short lived, with Scer\GAL4^elav.PU

(Zhang et al., 2015)

Phenotype Manifest In

dopaminergic neuron | adult stage, with Scer\GAL4^Ddc.PU

(Liu et al., 2015)

dopaminergic neuron | progressive, with Scer\GAL4^elav.PU

(Zhang et al., 2015)

dopaminergic PPL1 neuron, with Scer\GAL4^ple.PF

(Cunningham et al., 2018)

dopaminergic PPL1 neuron, with Scer\GAL4^ple.PU

(Babcock et al., 2015)

Detailed Description

Statement

Reference

Expressing Hsap\SNCA^UAS.cUa under the control of Scer\GAL4^ple.PF induces a progressive decrease in adult climbing capacity and results in 21-days old adults raised at 29[o]C exhibiting significantly fewer PPL1 neurons than age-matched controls; there is no obvious effect on lifespan.

(Cunningham et al., 2018)

Expression of Hsap\SNCA^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU does not lead to any significant decrease in the number of dopaminergic neurons from the PPM1/2 cluster in young (3 and 10 days old) adult flies.

(Song et al., 2017)

Expression of Hsap\SNCA^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU results in reduction of PPL1 dopaminergic neuron number, as compared with controls.

(Babcock et al., 2015)

Expression of Hsap\SNCA^Scer\UAS.cUa leads to reduced climbing ability (with either the Scer\GAL4^elav.PU or the Scer\GAL4^Ddc.PU driver) and loss of dopaminergic neurons (using the Scer\GAL4^Ddc.PU driver) in aged adult flies.

(Liu et al., 2015)

The lifespan of flies expressing Scer\GAL4^elav.PU>Hsap\SNCA^Scer\UAS.cUa is shorter than that of wild-type.

Aging flies expressing Scer\GAL4^elav.PU>Hsap\SNCA^Scer\UAS.cUa show impaired climbing ability and the progressive loss of dorsomedial dopaminergic neurons.

(Zhang et al., 2015)

Ectopic expression of Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF results in defects in locomotion and a significant decrease in the distance crawled by third instar larvae per unit of time compared to controls.

Ectopic expression of Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^elav.PU results in defects in negative geotaxis and significantly reduces climbing activity of adult flies compared to controls.

(Yin et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU has short lived phenotype, enhanceable by Lk6^UAS.cUa, Scer\GAL4^elav.PU

(Zhang et al., 2015)

Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU has abnormal neuroanatomy | progressive phenotype, enhanceable by Lk6^UAS.cUa, Scer\GAL4^elav.PU

(Zhang et al., 2015)

Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Lk6^UAS.cUa, Scer\GAL4^elav.PU

(Zhang et al., 2015)

Suppressed by

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PF has decreased cell number | adult stage phenotype, suppressible by st^UAS.Venus, Scer\GAL4^ple.PF

(Cunningham et al., 2018)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PF has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by st^UAS.Venus, Scer\GAL4^ple.PF

(Cunningham et al., 2018)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by comt^UAS.cGa, Scer\GAL4^ple.PU

(Babcock et al., 2015)

Hsap\SNCA^UAS.cUa, Scer\GAL4^C164 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab8^UASp.YFP, Scer\GAL4^C164

(Yin et al., 2014)

Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage phenotype, suppressible by Rab8^UASp.YFP, Scer\GAL4^elav.PU

(Yin et al., 2014)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PF has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab8^UASp.YFP, Scer\GAL4^ple.PF

(Yin et al., 2014)

Other

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has abnormal neuroanatomy | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has decreased cell number | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^KK100927 has abnormal neuroanatomy | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^KK100927 has decreased cell number | adult stage phenotype

(Song et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU has dopaminergic neuron | progressive phenotype, enhanceable by Lk6^UAS.cUa, Scer\GAL4^elav.PU

(Zhang et al., 2015)

Suppressed by

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PF has dopaminergic PPL1 neuron phenotype, suppressible by st^UAS.Venus, Scer\GAL4^ple.PF

(Cunningham et al., 2018)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPL1 neuron phenotype, suppressible by comt^UAS.cGa, Scer\GAL4^ple.PU

(Babcock et al., 2015)

Other

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM2 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM1 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^KK100927 has dopaminergic PPM2 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^KK100927 has dopaminergic PPM1 neuron | adult stage phenotype

(Song et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\SNCA^Scer\UAS.cUa with either aux^GD7187 or aux^KK100927 leads to significant decrease in the number of dopaminergic PPM1/2 neurons in the brains of young (3 and/or 10 days old) adult flies, which is not observed at this age when either of the transgenes is expressed alone.

(Song et al., 2017)

Co-expression of comt^Scer\UAS.cGa rescues the PPL1 dopaminergic neuron loss phenotype of flies expressing Hsap\SNCA^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU.

(Babcock et al., 2015)

The locomotion defects characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

The negative geotaxis defects characteristic for flies expressing Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^elav.PU can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the reduction in climbing activity of adult flies is significantly ameliorated.

(Yin et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SNCA^Scer\UAS.cUa

Hsap\SNCA^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (16)

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