FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rac1, Pak3
FlyBase ID
FBig0000044148
Interaction Network
Interactions Browser links
Rac1 network
Pak3 network
Reported Interactions
FBrf0187582-2.coIP.WB
Reference
Mentzel and Raabe, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rac1 

DmRAC1

bait

Myc tag

Pak3 

DmPAK3

prey

HA tag

Pak3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pak3 
PBD domain
mutation disrupting interaction
aa 29-31

H29L, H31L double mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mentzel and Raabe, 2005)

Pak3 binds to the activated form of Rac1.

(Mentzel and Raabe, 2005)

Pak3 binds to Rac1, Rac2 and weakly to Cdc42, but not to other RhoGTPases tested (Mtl, Rho1 or RhoL).

(Mentzel and Raabe, 2005)
FBrf0219579-1.coIP.WB
Reference
Duan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pak3 

DPak3

bait

V5 tag

Pak3 
Rac1 

Rac1

prey

activated Rac G12V mutant

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pak3 
PBD/AID domain
mutation disrupting interaction
aa 29-31

H29L, H31L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Duan et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)