FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hpo, mats
FlyBase ID
FBig0000098669
Interaction Network
Interactions Browser links
hpo network
mats network
Reported Interactions
FBrf0217990-11.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

enzyme

FLAG tag

hpo 

Hpo

enzyme

FLAG tag

hpo 
hpo 
mats 

Mats

enzyme target

GST tag, [32]P label

mats 

Mats

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0229674-4.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

hpo

enzyme

FLAG tag

hpo 

hpo

enzyme

FLAG tag

mats 

mats

enzyme target

GST tag

mats 

mats

enzyme target

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0237510-1.PD.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
mats 

Mats

bait

GST tag

hpo 

Hpo

prey

FLAG tag

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
linker domain
mutation decreasing interaction
aa 356,374,387,421,431aa 374,387,421,431aa 356,387,421,431aa 356,374,421,431aa 356,374,387,431

T356A,T374A,T387A,T421A,T431A

T374A,T387A,T421A,T431A

T356A,T387A,T421A,T431A

T356A,T374A,T421A,T431A

T356A,T374A,T387A,T431A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.

(Zheng et al., 2017)

There is no decrease in hpo-mats interaction when all putative phosphorylation sites in the hpo linker domain except T431 are mutated suggesting T431 is a major contributor to mats binding.

(Zheng et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)