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Citation
Vilmos, P., Bujna, A., Szuperák, M., Havelda, Z., Várallyay, E., Szabad, J., Kucerova, L., Somogyi, K., Kristó, I., Lukácsovich, T., Jankovics, F., Henn, L., Erdélyi, M. (2013). Viability, Longevity, and Egg Production of Drosophila melanogaster Are Regulated by the miR-282 microRNA.  Genetics 195(2): 469--480.
FlyBase ID
FBrf0222900
Publication Type
Research paper
Abstract

The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature microRNA of ∼25 bp. Our data strongly suggest the existence of an independent mir-282 gene conserved in holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript that influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target of miR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.

PubMed ID
PubMed Central ID
PMC3781974 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

P{UAS-mir-282.V} and P{Act5C-EGFP.rut3'UTR} insertions.
Vilmos, 2013.11.7, P{UAS-mir-282.V} and P{Act5C-EGFP.rut3'UTR} insertions. [FBrf0223277]

P{UASp-mir-282.V} construct and insertions.
Vilmos, 2013.11.7, P{UASp-mir-282.V} construct and insertions. [FBrf0223283]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference