Mobilization of P{RS3}CB-5453-3 resulting in a deletion of 1359 nucleotides that cover the entire mir-282 pre-miRNA region.
mir-282RSX homozygous adults display no visible phenotype, but have a shortened adult lifespan (~50% compared to controls) and semilethality (44% compared to controls) before reaching adult stage.
While mutant males display normal fertility, mutant females exhibit reduced egg production compared to controls, laying on average 55% less.
10 day old mutant females have germaria of elongated shape, but the numbers of germline stem cells and cysts are normal.
Transplantation of embryonic germ cells from mir-282RSX null embryos into ovoD1 females results in normal egg production in those females, indicating that somatic cells but not germ cells require normal mir-282 function.
Ovaries of 10-day old mir-282 females show normal apoptotic activity at checkpoints 8 and 12, but abnormal apoptosis in eggs after stage 12 with apparent accumulation of apoptotic debris in the proximal end of the oviduct.
The kinetics of reduction and recovery of egg yield after temporary starvation is similar in mir-282 and control females.
mir-282RSX is rescued by mir-282+t9.1
